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In vivo characterisation of fluorescent proteins in budding yeast
Fluorescent proteins (FPs) are widely used in many organisms, but are commonly characterised in vitro. However, the in vitro properties may poorly reflect in vivo performance. Therefore, we characterised 27 FPs in vivo using Saccharomyces cerevisiae as model organism. We linked the FPs via a T2A pep...
Autores principales: | Botman, Dennis, de Groot, Daan Hugo, Schmidt, Phillipp, Goedhart, Joachim, Teusink, Bas |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381139/ https://www.ncbi.nlm.nih.gov/pubmed/30783202 http://dx.doi.org/10.1038/s41598-019-38913-z |
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