Efficient system for upstream mRNA trans-splicing to generate covalent, head-to-tail, protein multimers

We present a plasmid-based system in which upstream trans-splicing efficiently generates mRNAs that encode head-to-tail protein multimers. In this system, trans-splicing occurs between one of two downstream splice donors in the sequence encoding a C-terminal V5 epitope tag and an upstream splice acc...

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Detalles Bibliográficos
Autores principales: Mitsuhashi, Hiroaki, Homma, Sachiko, Beermann, Mary Lou, Ishimaru, Satoshi, Takeda, Hayato, Yu, Bryant K., Liu, Kevin, Duraiswamy, Swetha, Boyce, Frederick M., Miller, Jeffrey Boone
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381186/
https://www.ncbi.nlm.nih.gov/pubmed/30783185
http://dx.doi.org/10.1038/s41598-018-36684-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381186/
https://www.ncbi.nlm.nih.gov/pubmed/30783185
http://dx.doi.org/10.1038/s41598-018-36684-7

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