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Efficient system for upstream mRNA trans-splicing to generate covalent, head-to-tail, protein multimers
We present a plasmid-based system in which upstream trans-splicing efficiently generates mRNAs that encode head-to-tail protein multimers. In this system, trans-splicing occurs between one of two downstream splice donors in the sequence encoding a C-terminal V5 epitope tag and an upstream splice acc...
| Autores principales: | , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381186/ https://www.ncbi.nlm.nih.gov/pubmed/30783185 http://dx.doi.org/10.1038/s41598-018-36684-7 |