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Targeted Next Generation Sequencing to study insert stability in genetically modified plants

The EU directive 2001/18/EC requires any genetically modified (GM) event to be stable. In the present work, a targeted Next-Generation Sequencing (NGS) approach using barcodes to specifically tag each individual DNA molecules during library preparation was implemented to detect mutations taking into...

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Detalles Bibliográficos
Autores principales: Boutigny, Anne-Laure, Barranger, Audrey, De Boisséson, Claire, Blanchard, Yannick, Rolland, Mathieu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381221/
https://www.ncbi.nlm.nih.gov/pubmed/30783176
http://dx.doi.org/10.1038/s41598-019-38701-9