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Ultrahigh-Throughput Multiplexing and Sequencing of >500-Base-Pair Amplicon Regions on the Illumina HiSeq 2500 Platform

Amplification, sequencing, and analysis of the 16S rRNA gene affords characterization of microbial community composition. As this tool has become more popular and amplicon-sequencing applications have grown in the total number of samples, growth in sample multiplexing is becoming necessary while mai...

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Detalles Bibliográficos
Autores principales: Holm, Johanna B., Humphrys, Michael S., Robinson, Courtney K., Settles, Matthew L., Ott, Sandra, Fu, Li, Yang, Hongqiu, Gajer, Pawel, He, Xin, McComb, Elias, Gravitt, Patti E., Ghanem, Khalil G., Brotman, Rebecca M., Ravel, Jacques
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381223/
https://www.ncbi.nlm.nih.gov/pubmed/30801027
http://dx.doi.org/10.1128/mSystems.00029-19