ZFP57 suppress proliferation of breast cancer cells through down-regulation of MEST-mediated Wnt/β-catenin signalling pathway

Activation of oncogenes by promoter hypomethylation plays an important role in tumorigenesis. Zinc finger protein 57 (ZFP57), a member of KRAB-ZFPs, could maintain DNA methylation in embryonic stem cells (ESCs), although its role and underlying mechanisms in breast cancer are not well understood. In...

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Autores principales: Chen, Lie, Wu, Xiaowei, Xie, Hui, Yao, Na, Xia, Yiqin, Ma, Ge, Qian, Mengjia, Ge, Han, Cui, Yangyang, Huang, Yue, Wang, Shui, Zheng, Mingjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382817/
https://www.ncbi.nlm.nih.gov/pubmed/30787268
http://dx.doi.org/10.1038/s41419-019-1335-5
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author Chen, Lie
Wu, Xiaowei
Xie, Hui
Yao, Na
Xia, Yiqin
Ma, Ge
Qian, Mengjia
Ge, Han
Cui, Yangyang
Huang, Yue
Wang, Shui
Zheng, Mingjie
author_facet Chen, Lie
Wu, Xiaowei
Xie, Hui
Yao, Na
Xia, Yiqin
Ma, Ge
Qian, Mengjia
Ge, Han
Cui, Yangyang
Huang, Yue
Wang, Shui
Zheng, Mingjie
author_sort Chen, Lie
collection PubMed
description Activation of oncogenes by promoter hypomethylation plays an important role in tumorigenesis. Zinc finger protein 57 (ZFP57), a member of KRAB-ZFPs, could maintain DNA methylation in embryonic stem cells (ESCs), although its role and underlying mechanisms in breast cancer are not well understood. In this study, we found that ZFP57 had low expression in breast cancer, and overexpression of ZFP57 could inhibit the proliferation of breast cancer cells by inhibiting the Wnt/β-catenin pathway. MEST was validated as the direct target gene of ZFP57 and MEST may be down-regulated by ZFP57 through conserving DNA methylation. Furthermore, overexpression of MEST could restore the tumour-suppressed and the Wnt/β-catenin pathway inactivated effects of ZFP57. ZFP57-MEST and the Wnt/β-catenin pathway axis are involved in breast tumorigenesis, which may represent a potential diagnostic biomarker, and provide a new insight into a novel therapeutic strategy for breast cancer patients.
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spelling pubmed-63828172019-02-21 ZFP57 suppress proliferation of breast cancer cells through down-regulation of MEST-mediated Wnt/β-catenin signalling pathway Chen, Lie Wu, Xiaowei Xie, Hui Yao, Na Xia, Yiqin Ma, Ge Qian, Mengjia Ge, Han Cui, Yangyang Huang, Yue Wang, Shui Zheng, Mingjie Cell Death Dis Article Activation of oncogenes by promoter hypomethylation plays an important role in tumorigenesis. Zinc finger protein 57 (ZFP57), a member of KRAB-ZFPs, could maintain DNA methylation in embryonic stem cells (ESCs), although its role and underlying mechanisms in breast cancer are not well understood. In this study, we found that ZFP57 had low expression in breast cancer, and overexpression of ZFP57 could inhibit the proliferation of breast cancer cells by inhibiting the Wnt/β-catenin pathway. MEST was validated as the direct target gene of ZFP57 and MEST may be down-regulated by ZFP57 through conserving DNA methylation. Furthermore, overexpression of MEST could restore the tumour-suppressed and the Wnt/β-catenin pathway inactivated effects of ZFP57. ZFP57-MEST and the Wnt/β-catenin pathway axis are involved in breast tumorigenesis, which may represent a potential diagnostic biomarker, and provide a new insight into a novel therapeutic strategy for breast cancer patients. Nature Publishing Group UK 2019-02-20 /pmc/articles/PMC6382817/ /pubmed/30787268 http://dx.doi.org/10.1038/s41419-019-1335-5 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Chen, Lie
Wu, Xiaowei
Xie, Hui
Yao, Na
Xia, Yiqin
Ma, Ge
Qian, Mengjia
Ge, Han
Cui, Yangyang
Huang, Yue
Wang, Shui
Zheng, Mingjie
ZFP57 suppress proliferation of breast cancer cells through down-regulation of MEST-mediated Wnt/β-catenin signalling pathway
title ZFP57 suppress proliferation of breast cancer cells through down-regulation of MEST-mediated Wnt/β-catenin signalling pathway
title_full ZFP57 suppress proliferation of breast cancer cells through down-regulation of MEST-mediated Wnt/β-catenin signalling pathway
title_fullStr ZFP57 suppress proliferation of breast cancer cells through down-regulation of MEST-mediated Wnt/β-catenin signalling pathway
title_full_unstemmed ZFP57 suppress proliferation of breast cancer cells through down-regulation of MEST-mediated Wnt/β-catenin signalling pathway
title_short ZFP57 suppress proliferation of breast cancer cells through down-regulation of MEST-mediated Wnt/β-catenin signalling pathway
title_sort zfp57 suppress proliferation of breast cancer cells through down-regulation of mest-mediated wnt/β-catenin signalling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382817/
https://www.ncbi.nlm.nih.gov/pubmed/30787268
http://dx.doi.org/10.1038/s41419-019-1335-5
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