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Continuous culture of Escherichia coli, under selective pressure by a novel antimicrobial complex, does not result in development of resistance

We attempted to generate de novo resistance to a newly described biocidal complex, ITC (iodo-thiocyanate complex), and to levofloxacin (LVX) in Escherichia coli ATCC 25922, by means of selective chemostat culture. We measured resistance by determining the minimum inhibitory concentrations (MICs) for...

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Autores principales: Tonoyan, Lilit, Fleming, Gerard T. A., Friel, Ruairi, O’Flaherty, Vincent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382887/
https://www.ncbi.nlm.nih.gov/pubmed/30787338
http://dx.doi.org/10.1038/s41598-019-38925-9
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author Tonoyan, Lilit
Fleming, Gerard T. A.
Friel, Ruairi
O’Flaherty, Vincent
author_facet Tonoyan, Lilit
Fleming, Gerard T. A.
Friel, Ruairi
O’Flaherty, Vincent
author_sort Tonoyan, Lilit
collection PubMed
description We attempted to generate de novo resistance to a newly described biocidal complex, ITC (iodo-thiocyanate complex), and to levofloxacin (LVX) in Escherichia coli ATCC 25922, by means of selective chemostat culture. We measured resistance by determining the minimum inhibitory concentrations (MICs) for these agents. E. coli underwent 20-day parallel adaptive evolution routes under no antimicrobial selection, and gradually increasing ITC and LVX selection pressure. Long-term exposure of E. coli to ITC did not induce resistance to ITC, or cross-resistance to LVX. No distinct mutational pattern was evidenced from whole-genome sequence (WGS)-based comparisons of ITC-challenged and unchallenged bacterial populations. Moreover, the exposed E. coli population could not survive a 2 × MIC challenge of ITC. By contrast, resistance to LVX was rapidly induced (on day 1 the MIC had increased 16-fold), selected for (by day 14 the MIC had increased 64-fold) and enriched with a highly characteristic genome mutational pattern. WGS of this evolving population revealed that the majority of mutations appeared in the genes of LVX target proteins (GyrA, ParC, ParE) and drug influx (OmpF). This study suggests that the usage of ITC may not trigger the emergence of facile resistance or cross-resistance, in contrast to common antibiotics.
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spelling pubmed-63828872019-02-25 Continuous culture of Escherichia coli, under selective pressure by a novel antimicrobial complex, does not result in development of resistance Tonoyan, Lilit Fleming, Gerard T. A. Friel, Ruairi O’Flaherty, Vincent Sci Rep Article We attempted to generate de novo resistance to a newly described biocidal complex, ITC (iodo-thiocyanate complex), and to levofloxacin (LVX) in Escherichia coli ATCC 25922, by means of selective chemostat culture. We measured resistance by determining the minimum inhibitory concentrations (MICs) for these agents. E. coli underwent 20-day parallel adaptive evolution routes under no antimicrobial selection, and gradually increasing ITC and LVX selection pressure. Long-term exposure of E. coli to ITC did not induce resistance to ITC, or cross-resistance to LVX. No distinct mutational pattern was evidenced from whole-genome sequence (WGS)-based comparisons of ITC-challenged and unchallenged bacterial populations. Moreover, the exposed E. coli population could not survive a 2 × MIC challenge of ITC. By contrast, resistance to LVX was rapidly induced (on day 1 the MIC had increased 16-fold), selected for (by day 14 the MIC had increased 64-fold) and enriched with a highly characteristic genome mutational pattern. WGS of this evolving population revealed that the majority of mutations appeared in the genes of LVX target proteins (GyrA, ParC, ParE) and drug influx (OmpF). This study suggests that the usage of ITC may not trigger the emergence of facile resistance or cross-resistance, in contrast to common antibiotics. Nature Publishing Group UK 2019-02-20 /pmc/articles/PMC6382887/ /pubmed/30787338 http://dx.doi.org/10.1038/s41598-019-38925-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Tonoyan, Lilit
Fleming, Gerard T. A.
Friel, Ruairi
O’Flaherty, Vincent
Continuous culture of Escherichia coli, under selective pressure by a novel antimicrobial complex, does not result in development of resistance
title Continuous culture of Escherichia coli, under selective pressure by a novel antimicrobial complex, does not result in development of resistance
title_full Continuous culture of Escherichia coli, under selective pressure by a novel antimicrobial complex, does not result in development of resistance
title_fullStr Continuous culture of Escherichia coli, under selective pressure by a novel antimicrobial complex, does not result in development of resistance
title_full_unstemmed Continuous culture of Escherichia coli, under selective pressure by a novel antimicrobial complex, does not result in development of resistance
title_short Continuous culture of Escherichia coli, under selective pressure by a novel antimicrobial complex, does not result in development of resistance
title_sort continuous culture of escherichia coli, under selective pressure by a novel antimicrobial complex, does not result in development of resistance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382887/
https://www.ncbi.nlm.nih.gov/pubmed/30787338
http://dx.doi.org/10.1038/s41598-019-38925-9
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