Effects of Lid 1 Mutagenesis on Lid Displacement, Catalytic Performances and Thermostability of Cold-active Pseudomonas AMS8 Lipase in Toluene

Pseudomonas fluorescens AMS8 lipase lid 1 structure is rigid and holds unclear roles due to the absence of solvent-interactions. Lid 1 region was stabilized by 17 hydrogen bond linkages and displayed lower mean hydrophobicity (0.596) compared to MIS38 lipase. Mutating lid 1 residues, Thr-52 and Gly-...

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Detalles Bibliográficos
Autores principales: Yaacob, Norhayati, Ahmad Kamarudin, Nor Hafizah, Leow, Adam Thean Chor, Salleh, Abu Bakar, Rahman, Raja Noor Zaliha Raja Abd, Ali, Mohd Shukuri Mohamad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Network of Computational and Structural Biotechnology 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383135/
https://www.ncbi.nlm.nih.gov/pubmed/30828413
http://dx.doi.org/10.1016/j.csbj.2019.01.005

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383135/
https://www.ncbi.nlm.nih.gov/pubmed/30828413
http://dx.doi.org/10.1016/j.csbj.2019.01.005

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