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Testing for goodness rather than lack of fit of an X–chromosomal SNP to the Hardy-Weinberg model
The problem of checking the genotype distribution obtained for some diallelic marker for compatibility with the Hardy-Weinberg equilibrium (HWE) condition arises also for loci on the X chromosome. The possible genotypes depend on the sex of the individual in this case: for females, the genotype dist...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383894/ https://www.ncbi.nlm.nih.gov/pubmed/30789927 http://dx.doi.org/10.1371/journal.pone.0212344 |
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author | Wellek, Stefan Ziegler, Andreas |
author_facet | Wellek, Stefan Ziegler, Andreas |
author_sort | Wellek, Stefan |
collection | PubMed |
description | The problem of checking the genotype distribution obtained for some diallelic marker for compatibility with the Hardy-Weinberg equilibrium (HWE) condition arises also for loci on the X chromosome. The possible genotypes depend on the sex of the individual in this case: for females, the genotype distribution is trinomial, as in the case of an autosomal locus, whereas a binomial proportion is observed for males. Like in genetic association studies with autosomal SNPs, interest is typically in establishing approximate compatibility of the observed genotype frequencies with HWE. This requires to replace traditional methods tailored for detecting lack of fit to the model with an equivalence testing procedure to be derived by treating approximate compatibility with the model as the alternative hypothesis. The test constructed here is based on an upper confidence bound and a simple to interpret combined measure of distance between true and HWE conforming genotype distributions in female and male subjects. A particular focus of the paper is on the derivation of the asymptotic distribution of the test statistic under null alternatives which is not of the usual Gaussian form. A closed sample size formula is also provided and shown to behave satisfactorily in terms of the approximation error. |
format | Online Article Text |
id | pubmed-6383894 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-63838942019-03-09 Testing for goodness rather than lack of fit of an X–chromosomal SNP to the Hardy-Weinberg model Wellek, Stefan Ziegler, Andreas PLoS One Research Article The problem of checking the genotype distribution obtained for some diallelic marker for compatibility with the Hardy-Weinberg equilibrium (HWE) condition arises also for loci on the X chromosome. The possible genotypes depend on the sex of the individual in this case: for females, the genotype distribution is trinomial, as in the case of an autosomal locus, whereas a binomial proportion is observed for males. Like in genetic association studies with autosomal SNPs, interest is typically in establishing approximate compatibility of the observed genotype frequencies with HWE. This requires to replace traditional methods tailored for detecting lack of fit to the model with an equivalence testing procedure to be derived by treating approximate compatibility with the model as the alternative hypothesis. The test constructed here is based on an upper confidence bound and a simple to interpret combined measure of distance between true and HWE conforming genotype distributions in female and male subjects. A particular focus of the paper is on the derivation of the asymptotic distribution of the test statistic under null alternatives which is not of the usual Gaussian form. A closed sample size formula is also provided and shown to behave satisfactorily in terms of the approximation error. Public Library of Science 2019-02-21 /pmc/articles/PMC6383894/ /pubmed/30789927 http://dx.doi.org/10.1371/journal.pone.0212344 Text en © 2019 Wellek, Ziegler http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wellek, Stefan Ziegler, Andreas Testing for goodness rather than lack of fit of an X–chromosomal SNP to the Hardy-Weinberg model |
title | Testing for goodness rather than lack of fit of an X–chromosomal SNP to the Hardy-Weinberg model |
title_full | Testing for goodness rather than lack of fit of an X–chromosomal SNP to the Hardy-Weinberg model |
title_fullStr | Testing for goodness rather than lack of fit of an X–chromosomal SNP to the Hardy-Weinberg model |
title_full_unstemmed | Testing for goodness rather than lack of fit of an X–chromosomal SNP to the Hardy-Weinberg model |
title_short | Testing for goodness rather than lack of fit of an X–chromosomal SNP to the Hardy-Weinberg model |
title_sort | testing for goodness rather than lack of fit of an x–chromosomal snp to the hardy-weinberg model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383894/ https://www.ncbi.nlm.nih.gov/pubmed/30789927 http://dx.doi.org/10.1371/journal.pone.0212344 |
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