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Astilbin Inhibits the Activity of Sortase A from Streptococcus mutans

Streptococcus mutans (S. mutans) is the primary etiological agent of dental caries. The S. mutans enzyme sortase A (SrtA) is responsible for anchoring bacterial cell wall surface proteins involved in host cell attachment and biofilm formation. Thus, SrtA is an attractive target for inhibiting dental...

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Detalles Bibliográficos
Autores principales: Wang, Junxian, Shi, Yan, Jing, Shisong, Dong, Haisi, Wang, Dacheng, Wang, Tiedong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384703/
https://www.ncbi.nlm.nih.gov/pubmed/30696091
http://dx.doi.org/10.3390/molecules24030465
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author Wang, Junxian
Shi, Yan
Jing, Shisong
Dong, Haisi
Wang, Dacheng
Wang, Tiedong
author_facet Wang, Junxian
Shi, Yan
Jing, Shisong
Dong, Haisi
Wang, Dacheng
Wang, Tiedong
author_sort Wang, Junxian
collection PubMed
description Streptococcus mutans (S. mutans) is the primary etiological agent of dental caries. The S. mutans enzyme sortase A (SrtA) is responsible for anchoring bacterial cell wall surface proteins involved in host cell attachment and biofilm formation. Thus, SrtA is an attractive target for inhibiting dental caries caused by S. mutans-associated acid fermentation. In this study, we observed that astilbin, a flavanone compound extracted from Rhizoma Smilacis Glabrae, has potent inhibitory activity against the S. mutans SrtA, with an IC(50) of 7.5 μg/mL. In addition, astilbin was proven to reduce the formation of biofilm while without affecting the growth of S. mutans. The results of a molecular dynamics simulation and a mutation analysis revealed that the Arg213, Leu111, and Leu116 of SrtA are important for the interaction between SrtA and astilbin. The results of this study demonstrate the potential of using astilbin as a nonbactericidal agent to modulate pathogenicity of S. mutans by inhibiting the activity of SrtA.
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spelling pubmed-63847032019-02-23 Astilbin Inhibits the Activity of Sortase A from Streptococcus mutans Wang, Junxian Shi, Yan Jing, Shisong Dong, Haisi Wang, Dacheng Wang, Tiedong Molecules Article Streptococcus mutans (S. mutans) is the primary etiological agent of dental caries. The S. mutans enzyme sortase A (SrtA) is responsible for anchoring bacterial cell wall surface proteins involved in host cell attachment and biofilm formation. Thus, SrtA is an attractive target for inhibiting dental caries caused by S. mutans-associated acid fermentation. In this study, we observed that astilbin, a flavanone compound extracted from Rhizoma Smilacis Glabrae, has potent inhibitory activity against the S. mutans SrtA, with an IC(50) of 7.5 μg/mL. In addition, astilbin was proven to reduce the formation of biofilm while without affecting the growth of S. mutans. The results of a molecular dynamics simulation and a mutation analysis revealed that the Arg213, Leu111, and Leu116 of SrtA are important for the interaction between SrtA and astilbin. The results of this study demonstrate the potential of using astilbin as a nonbactericidal agent to modulate pathogenicity of S. mutans by inhibiting the activity of SrtA. MDPI 2019-01-28 /pmc/articles/PMC6384703/ /pubmed/30696091 http://dx.doi.org/10.3390/molecules24030465 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Junxian
Shi, Yan
Jing, Shisong
Dong, Haisi
Wang, Dacheng
Wang, Tiedong
Astilbin Inhibits the Activity of Sortase A from Streptococcus mutans
title Astilbin Inhibits the Activity of Sortase A from Streptococcus mutans
title_full Astilbin Inhibits the Activity of Sortase A from Streptococcus mutans
title_fullStr Astilbin Inhibits the Activity of Sortase A from Streptococcus mutans
title_full_unstemmed Astilbin Inhibits the Activity of Sortase A from Streptococcus mutans
title_short Astilbin Inhibits the Activity of Sortase A from Streptococcus mutans
title_sort astilbin inhibits the activity of sortase a from streptococcus mutans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384703/
https://www.ncbi.nlm.nih.gov/pubmed/30696091
http://dx.doi.org/10.3390/molecules24030465
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