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Factors affecting genetic transformation by particle bombardment of the prickly pear cactus (O. ficus-indica)

In the present study, a novel transformation protocol for Opuntia ficus-indica was generated by means of particle bombardment. The best conditions obtained were: 900 psi rupture disk pressure, 8 cm microprojectile travel distance, and 4 h of exposition to 0.2 M mannitol. For all experiments, gold pa...

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Detalles Bibliográficos
Autores principales: Angulo-Bejarano, Paola Isabel, Sharma, Ashutosh, Paredes-López, Octavio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6385058/
https://www.ncbi.nlm.nih.gov/pubmed/30800609
http://dx.doi.org/10.1007/s13205-019-1627-6