Cell-Free Protein Synthesis Using S30 Extracts from Escherichia coli RFzero Strains for Efficient Incorporation of Non-Natural Amino Acids into Proteins

Cell-free protein synthesis is useful for synthesizing difficult targets. The site-specific incorporation of non-natural amino acids into proteins is a powerful protein engineering method. In this study, we optimized the protocol for cell extract preparation from the Escherichia coli strain RFzero-i...

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Detalles Bibliográficos
Autores principales: Adachi, Jiro, Katsura, Kazushige, Seki, Eiko, Takemoto, Chie, Shirouzu, Mikako, Terada, Takaho, Mukai, Takahito, Sakamoto, Kensaku, Yokoyama, Shigeyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387211/
https://www.ncbi.nlm.nih.gov/pubmed/30678326
http://dx.doi.org/10.3390/ijms20030492

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387211/
https://www.ncbi.nlm.nih.gov/pubmed/30678326
http://dx.doi.org/10.3390/ijms20030492

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