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Cell-Free Protein Synthesis Using S30 Extracts from Escherichia coli RFzero Strains for Efficient Incorporation of Non-Natural Amino Acids into Proteins
Cell-free protein synthesis is useful for synthesizing difficult targets. The site-specific incorporation of non-natural amino acids into proteins is a powerful protein engineering method. In this study, we optimized the protocol for cell extract preparation from the Escherichia coli strain RFzero-i...
Autores principales: | Adachi, Jiro, Katsura, Kazushige, Seki, Eiko, Takemoto, Chie, Shirouzu, Mikako, Terada, Takaho, Mukai, Takahito, Sakamoto, Kensaku, Yokoyama, Shigeyuki |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387211/ https://www.ncbi.nlm.nih.gov/pubmed/30678326 http://dx.doi.org/10.3390/ijms20030492 |
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