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Cross-site comparison of ribosomal depletion kits for Illumina RNAseq library construction

BACKGROUND: Ribosomal RNA (rRNA) comprises at least 90% of total RNA extracted from mammalian tissue or cell line samples. Informative transcriptional profiling using massively parallel sequencing technologies requires either enrichment of mature poly-adenylated transcripts or targeted depletion of...

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Detalles Bibliográficos
Autores principales: Herbert, Zachary T., Kershner, Jamie P., Butty, Vincent L., Thimmapuram, Jyothi, Choudhari, Sulbha, Alekseyev, Yuriy O., Fan, Jun, Podnar, Jessica W., Wilcox, Edward, Gipson, Jenny, Gillaspy, Allison, Jepsen, Kristen, BonDurant, Sandra Splinter, Morris, Krystalynne, Berkeley, Maura, LeClerc, Ashley, Simpson, Stephen D., Sommerville, Gary, Grimmett, Leslie, Adams, Marie, Levine, Stuart S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389247/
https://www.ncbi.nlm.nih.gov/pubmed/29703133
http://dx.doi.org/10.1186/s12864-018-4585-1