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Beyond antibody engineering: directed evolution of alternative binding scaffolds and enzymes using yeast surface display
Pioneered exactly 20 years ago, yeast surface display (YSD) continues to take a major role in protein engineering among the high-throughput display methodologies that have been developed to date. The classical yeast display technology relies on tethering an engineered protein to the cell wall by gen...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389260/ https://www.ncbi.nlm.nih.gov/pubmed/29482656 http://dx.doi.org/10.1186/s12934-018-0881-3 |