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Beyond antibody engineering: directed evolution of alternative binding scaffolds and enzymes using yeast surface display

Pioneered exactly 20 years ago, yeast surface display (YSD) continues to take a major role in protein engineering among the high-throughput display methodologies that have been developed to date. The classical yeast display technology relies on tethering an engineered protein to the cell wall by gen...

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Detalles Bibliográficos
Autores principales: Könning, Doreen, Kolmar, Harald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389260/
https://www.ncbi.nlm.nih.gov/pubmed/29482656
http://dx.doi.org/10.1186/s12934-018-0881-3