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A Novel Method of Isolating Myofibrils From Primary Cardiomyocyte Culture Suitable for Myofibril Mechanical Study

Myofibril based mechanical studies allow evaluation of sarcomeric protein function. We describe a novel method of obtaining myofibrils from primary cardiomyocyte culture. Adult rat ventricular myocytes (ARVMs) were obtained by enzymatic digestion and maintained in serum free condition. ARVMs were ho...

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Detalles Bibliográficos
Autores principales: Woulfe, Kathleen C., Ferrara, Claudia, Pioner, Jose Manuel, Mahaffey, Jennifer H., Coppini, Raffaele, Scellini, Beatrice, Ferrantini, Cecilia, Piroddi, Nicoletta, Tesi, Chiari, Poggesi, Corrado, Jeong, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389618/
https://www.ncbi.nlm.nih.gov/pubmed/30838216
http://dx.doi.org/10.3389/fcvm.2019.00012