A complex interaction between pre-harvest and post-harvest factors determines fresh-cut melon quality and aroma

Melons are prized for their characteristic aroma, however, pre-harvest growth, stage of ripening at harvest, post-harvest processing and storage conditions lead to quality changes in fresh-cut fruit. We considered changes in metabolites and gene expression over 14 days storage to assess underlying m...

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Autores principales: Spadafora, Natasha D., Cocetta, Giacomo, Cavaiuolo, Marina, Bulgari, Roberta, Dhorajiwala, Rakhee, Ferrante, Antonio, Spinardi, Anna, Rogers, Hilary J., Müller, Carsten T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391468/
https://www.ncbi.nlm.nih.gov/pubmed/30808957
http://dx.doi.org/10.1038/s41598-019-39196-0
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author Spadafora, Natasha D.
Cocetta, Giacomo
Cavaiuolo, Marina
Bulgari, Roberta
Dhorajiwala, Rakhee
Ferrante, Antonio
Spinardi, Anna
Rogers, Hilary J.
Müller, Carsten T.
author_facet Spadafora, Natasha D.
Cocetta, Giacomo
Cavaiuolo, Marina
Bulgari, Roberta
Dhorajiwala, Rakhee
Ferrante, Antonio
Spinardi, Anna
Rogers, Hilary J.
Müller, Carsten T.
author_sort Spadafora, Natasha D.
collection PubMed
description Melons are prized for their characteristic aroma, however, pre-harvest growth, stage of ripening at harvest, post-harvest processing and storage conditions lead to quality changes in fresh-cut fruit. We considered changes in metabolites and gene expression over 14 days storage to assess underlying mechanisms and identify potential quality markers. Overall, 99 volatile organic compounds (VOCs) were detected and VOC profiles discriminated between two melon seasons, cut-size, storage temperatures and storage time, although season affected their discriminatory power. Abundance of two VOCs fell rapidly and was not associated with cut size, indicating their use as markers for early changes post-processing. Non-acetate to acetate ester ratio differed between the seasons and correlated with changes in alcohol acyl-transferase (CmAAT1) gene expression. Furthermore, CmAAT1 expression clustered with two ester VOCs that may be potential new products of this enzyme. Season also strongly affected post-harvest sugar content, most likely attributable to meteorological differences during growth. Storage temperature and cut size affected expression of transcription factors ERF71, ERF106, and TINY, whose expression generally rose during storage, probably related to increased stress. Thus, although time × temperature of storage are key factors, pre-harvest conditions and fruit processing impact significantly gene expression and aroma loss post-harvest.
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spelling pubmed-63914682019-03-01 A complex interaction between pre-harvest and post-harvest factors determines fresh-cut melon quality and aroma Spadafora, Natasha D. Cocetta, Giacomo Cavaiuolo, Marina Bulgari, Roberta Dhorajiwala, Rakhee Ferrante, Antonio Spinardi, Anna Rogers, Hilary J. Müller, Carsten T. Sci Rep Article Melons are prized for their characteristic aroma, however, pre-harvest growth, stage of ripening at harvest, post-harvest processing and storage conditions lead to quality changes in fresh-cut fruit. We considered changes in metabolites and gene expression over 14 days storage to assess underlying mechanisms and identify potential quality markers. Overall, 99 volatile organic compounds (VOCs) were detected and VOC profiles discriminated between two melon seasons, cut-size, storage temperatures and storage time, although season affected their discriminatory power. Abundance of two VOCs fell rapidly and was not associated with cut size, indicating their use as markers for early changes post-processing. Non-acetate to acetate ester ratio differed between the seasons and correlated with changes in alcohol acyl-transferase (CmAAT1) gene expression. Furthermore, CmAAT1 expression clustered with two ester VOCs that may be potential new products of this enzyme. Season also strongly affected post-harvest sugar content, most likely attributable to meteorological differences during growth. Storage temperature and cut size affected expression of transcription factors ERF71, ERF106, and TINY, whose expression generally rose during storage, probably related to increased stress. Thus, although time × temperature of storage are key factors, pre-harvest conditions and fruit processing impact significantly gene expression and aroma loss post-harvest. Nature Publishing Group UK 2019-02-26 /pmc/articles/PMC6391468/ /pubmed/30808957 http://dx.doi.org/10.1038/s41598-019-39196-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Spadafora, Natasha D.
Cocetta, Giacomo
Cavaiuolo, Marina
Bulgari, Roberta
Dhorajiwala, Rakhee
Ferrante, Antonio
Spinardi, Anna
Rogers, Hilary J.
Müller, Carsten T.
A complex interaction between pre-harvest and post-harvest factors determines fresh-cut melon quality and aroma
title A complex interaction between pre-harvest and post-harvest factors determines fresh-cut melon quality and aroma
title_full A complex interaction between pre-harvest and post-harvest factors determines fresh-cut melon quality and aroma
title_fullStr A complex interaction between pre-harvest and post-harvest factors determines fresh-cut melon quality and aroma
title_full_unstemmed A complex interaction between pre-harvest and post-harvest factors determines fresh-cut melon quality and aroma
title_short A complex interaction between pre-harvest and post-harvest factors determines fresh-cut melon quality and aroma
title_sort complex interaction between pre-harvest and post-harvest factors determines fresh-cut melon quality and aroma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391468/
https://www.ncbi.nlm.nih.gov/pubmed/30808957
http://dx.doi.org/10.1038/s41598-019-39196-0
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