Tightly-orchestrated rearrangements govern catalytic center assembly of the ribosome

The catalytic activity of the ribosome is mediated by RNA, yet proteins are essential for the function of the peptidyl transferase center (PTC). In eukaryotes, final assembly of the PTC occurs in the cytoplasm by insertion of the ribosomal protein Rpl10 (uL16). We determine structures of six interme...

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Autores principales: Zhou, Yi, Musalgaonkar, Sharmishtha, Johnson, Arlen W., Taylor, David W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393466/
https://www.ncbi.nlm.nih.gov/pubmed/30814529
http://dx.doi.org/10.1038/s41467-019-08880-0
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author Zhou, Yi
Musalgaonkar, Sharmishtha
Johnson, Arlen W.
Taylor, David W.
author_facet Zhou, Yi
Musalgaonkar, Sharmishtha
Johnson, Arlen W.
Taylor, David W.
author_sort Zhou, Yi
collection PubMed
description The catalytic activity of the ribosome is mediated by RNA, yet proteins are essential for the function of the peptidyl transferase center (PTC). In eukaryotes, final assembly of the PTC occurs in the cytoplasm by insertion of the ribosomal protein Rpl10 (uL16). We determine structures of six intermediates in late nuclear and cytoplasmic maturation of the large subunit that reveal a tightly-choreographed sequence of protein and RNA rearrangements controlling the insertion of Rpl10. We also determine the structure of the biogenesis factor Yvh1 and show how it promotes assembly of the P stalk, a critical element for recruitment of GTPases that drive translation. Together, our structures provide a blueprint for final assembly of a functional ribosome.
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spelling pubmed-63934662019-03-01 Tightly-orchestrated rearrangements govern catalytic center assembly of the ribosome Zhou, Yi Musalgaonkar, Sharmishtha Johnson, Arlen W. Taylor, David W. Nat Commun Article The catalytic activity of the ribosome is mediated by RNA, yet proteins are essential for the function of the peptidyl transferase center (PTC). In eukaryotes, final assembly of the PTC occurs in the cytoplasm by insertion of the ribosomal protein Rpl10 (uL16). We determine structures of six intermediates in late nuclear and cytoplasmic maturation of the large subunit that reveal a tightly-choreographed sequence of protein and RNA rearrangements controlling the insertion of Rpl10. We also determine the structure of the biogenesis factor Yvh1 and show how it promotes assembly of the P stalk, a critical element for recruitment of GTPases that drive translation. Together, our structures provide a blueprint for final assembly of a functional ribosome. Nature Publishing Group UK 2019-02-27 /pmc/articles/PMC6393466/ /pubmed/30814529 http://dx.doi.org/10.1038/s41467-019-08880-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhou, Yi
Musalgaonkar, Sharmishtha
Johnson, Arlen W.
Taylor, David W.
Tightly-orchestrated rearrangements govern catalytic center assembly of the ribosome
title Tightly-orchestrated rearrangements govern catalytic center assembly of the ribosome
title_full Tightly-orchestrated rearrangements govern catalytic center assembly of the ribosome
title_fullStr Tightly-orchestrated rearrangements govern catalytic center assembly of the ribosome
title_full_unstemmed Tightly-orchestrated rearrangements govern catalytic center assembly of the ribosome
title_short Tightly-orchestrated rearrangements govern catalytic center assembly of the ribosome
title_sort tightly-orchestrated rearrangements govern catalytic center assembly of the ribosome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393466/
https://www.ncbi.nlm.nih.gov/pubmed/30814529
http://dx.doi.org/10.1038/s41467-019-08880-0
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AT taylordavidw tightlyorchestratedrearrangementsgoverncatalyticcenterassemblyoftheribosome

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