Nuclear import of Xenopus egg extract components into cultured cells for reprogramming purposes: a case study on goldfish fin cells

Reprogramming of cultured cells using Xenopus egg extract involves controlling four major steps: plasma membrane permeabilization, egg factors import into the nucleus, membrane resealing, and cell proliferation. Using propidium iodide to assess plasma membrane permeability, we established that 90% o...

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Detalles Bibliográficos
Autores principales: Chênais, Nathalie, Lorca, Thierry, Morin, Nathalie, Guillet, Brigitte, Rime, Hélène, Le Bail, Pierre-Yves, Labbé, Catherine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393519/
https://www.ncbi.nlm.nih.gov/pubmed/30814557
http://dx.doi.org/10.1038/s41598-019-39500-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393519/
https://www.ncbi.nlm.nih.gov/pubmed/30814557
http://dx.doi.org/10.1038/s41598-019-39500-y

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