Cargando…
Optimization and Designing of Amikacin-loaded Poly D, L-Lactide-co-glycolide Nanoparticles for Effective and Sustained Drug Delivery
PURPOSE: Amikacin, a water-soluble aminoglycoside antibiotic used to treat gram-negative bacillary infections, is a Biopharmaceutics Classification System class III drug having poor permeability and short half-life. It is given parenterally, which limits its use in patients warranting “at-home care....
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6394161/ https://www.ncbi.nlm.nih.gov/pubmed/30906144 http://dx.doi.org/10.4103/jpbs.JPBS_60_18 |
_version_ | 1783398837806694400 |
---|---|
author | Fatima, Saman Panda, Amulya K. Talegaonkar, Sushma Iqbal, Zeenat Ahmad, Farhan J. |
author_facet | Fatima, Saman Panda, Amulya K. Talegaonkar, Sushma Iqbal, Zeenat Ahmad, Farhan J. |
author_sort | Fatima, Saman |
collection | PubMed |
description | PURPOSE: Amikacin, a water-soluble aminoglycoside antibiotic used to treat gram-negative bacillary infections, is a Biopharmaceutics Classification System class III drug having poor permeability and short half-life. It is given parenterally, which limits its use in patients warranting “at-home care.” An oral drug delivery of amikacin is, therefore, imminent. AIM: This work focused on establishing poly d, l-lactide-co-glycolide (PLGA)-based nanoparticles of amikacin with consolidated pharmaceutical attributes capable of circumventing gastrointestinal tract membrane barriers and promoting oral administration of the drug. The partied attributes are suggestive of enhanced uptake of the drug via Peyer’s patches overlaying small intestine and support successful oral delivery. MATERIALS AND METHODS: To have a robust delivery system, a statistical Box–Behnken experimental design was used and formulation parameters such as homogenization time, probe sonication time, and drug/polymer ratio of amikacin-loaded PLGA nanoparticles (A-NPs) for obtaining monodispersed nanoparticles of adequate size and high drug loading were optimized. RESULTS: The model suggested to use the optimum homogenization time, probe sonication time, and drug/polymer ratio as 30 s, 120 s, and 1:10, respectively. Under these formulation conditions, the particle size was found to be 260.3 nm and the drug loading was 3.645%. CONCLUSION: Biodegradable PLGA nanoparticulate systems with high payload, optimum size, and low polydispersity index will ensure successful uptake and ultimately leading to better bioavailability. Hence, under the aforementioned optimized conditions, the A-NPs prepared had particle size of 260.3 nm, which is appreciable for its permeability across small intestine, and drug loading of 3.645%. |
format | Online Article Text |
id | pubmed-6394161 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-63941612019-03-22 Optimization and Designing of Amikacin-loaded Poly D, L-Lactide-co-glycolide Nanoparticles for Effective and Sustained Drug Delivery Fatima, Saman Panda, Amulya K. Talegaonkar, Sushma Iqbal, Zeenat Ahmad, Farhan J. J Pharm Bioallied Sci Original Article PURPOSE: Amikacin, a water-soluble aminoglycoside antibiotic used to treat gram-negative bacillary infections, is a Biopharmaceutics Classification System class III drug having poor permeability and short half-life. It is given parenterally, which limits its use in patients warranting “at-home care.” An oral drug delivery of amikacin is, therefore, imminent. AIM: This work focused on establishing poly d, l-lactide-co-glycolide (PLGA)-based nanoparticles of amikacin with consolidated pharmaceutical attributes capable of circumventing gastrointestinal tract membrane barriers and promoting oral administration of the drug. The partied attributes are suggestive of enhanced uptake of the drug via Peyer’s patches overlaying small intestine and support successful oral delivery. MATERIALS AND METHODS: To have a robust delivery system, a statistical Box–Behnken experimental design was used and formulation parameters such as homogenization time, probe sonication time, and drug/polymer ratio of amikacin-loaded PLGA nanoparticles (A-NPs) for obtaining monodispersed nanoparticles of adequate size and high drug loading were optimized. RESULTS: The model suggested to use the optimum homogenization time, probe sonication time, and drug/polymer ratio as 30 s, 120 s, and 1:10, respectively. Under these formulation conditions, the particle size was found to be 260.3 nm and the drug loading was 3.645%. CONCLUSION: Biodegradable PLGA nanoparticulate systems with high payload, optimum size, and low polydispersity index will ensure successful uptake and ultimately leading to better bioavailability. Hence, under the aforementioned optimized conditions, the A-NPs prepared had particle size of 260.3 nm, which is appreciable for its permeability across small intestine, and drug loading of 3.645%. Medknow Publications & Media Pvt Ltd 2019 /pmc/articles/PMC6394161/ /pubmed/30906144 http://dx.doi.org/10.4103/jpbs.JPBS_60_18 Text en Copyright: © 2019 Journal of Pharmacy and Bioallied Sciences http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Fatima, Saman Panda, Amulya K. Talegaonkar, Sushma Iqbal, Zeenat Ahmad, Farhan J. Optimization and Designing of Amikacin-loaded Poly D, L-Lactide-co-glycolide Nanoparticles for Effective and Sustained Drug Delivery |
title | Optimization and Designing of Amikacin-loaded Poly D, L-Lactide-co-glycolide Nanoparticles for Effective and Sustained Drug Delivery |
title_full | Optimization and Designing of Amikacin-loaded Poly D, L-Lactide-co-glycolide Nanoparticles for Effective and Sustained Drug Delivery |
title_fullStr | Optimization and Designing of Amikacin-loaded Poly D, L-Lactide-co-glycolide Nanoparticles for Effective and Sustained Drug Delivery |
title_full_unstemmed | Optimization and Designing of Amikacin-loaded Poly D, L-Lactide-co-glycolide Nanoparticles for Effective and Sustained Drug Delivery |
title_short | Optimization and Designing of Amikacin-loaded Poly D, L-Lactide-co-glycolide Nanoparticles for Effective and Sustained Drug Delivery |
title_sort | optimization and designing of amikacin-loaded poly d, l-lactide-co-glycolide nanoparticles for effective and sustained drug delivery |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6394161/ https://www.ncbi.nlm.nih.gov/pubmed/30906144 http://dx.doi.org/10.4103/jpbs.JPBS_60_18 |
work_keys_str_mv | AT fatimasaman optimizationanddesigningofamikacinloadedpolydllactidecoglycolidenanoparticlesforeffectiveandsustaineddrugdelivery AT pandaamulyak optimizationanddesigningofamikacinloadedpolydllactidecoglycolidenanoparticlesforeffectiveandsustaineddrugdelivery AT talegaonkarsushma optimizationanddesigningofamikacinloadedpolydllactidecoglycolidenanoparticlesforeffectiveandsustaineddrugdelivery AT iqbalzeenat optimizationanddesigningofamikacinloadedpolydllactidecoglycolidenanoparticlesforeffectiveandsustaineddrugdelivery AT ahmadfarhanj optimizationanddesigningofamikacinloadedpolydllactidecoglycolidenanoparticlesforeffectiveandsustaineddrugdelivery |