Isolation of gene-edited cells via knock-in of short glycophosphatidylinositol-anchored epitope tags

We describe Surface Oligopeptide knock-in for Rapid Target Selection (SORTS), a novel method to select mammalian cells with precise genome modifications that does not rely on cell cloning. SORTS is designed to disrupt the target gene with an expression cassette encoding an epitope tag embedded into...

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Detalles Bibliográficos
Autores principales: Zotova, Anastasia, Pichugin, Alexey, Atemasova, Anastasia, Knyazhanskaya, Ekaterina, Lopatukhina, Elena, Mitkin, Nikita, Holmuhamedov, Ekhson, Gottikh, Marina, Kuprash, Dmitry, Filatov, Alexander, Mazurov, Dmitriy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395743/
https://www.ncbi.nlm.nih.gov/pubmed/30816313
http://dx.doi.org/10.1038/s41598-019-40219-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395743/
https://www.ncbi.nlm.nih.gov/pubmed/30816313
http://dx.doi.org/10.1038/s41598-019-40219-z

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