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Promoter RNA sequencing (PRSeq) for the massive and quantitative promoter analysis in vitro

Analysis of promoter strength and specificity is important for understanding and engineering gene regulation. Here, we report an in vitro promoter analysis method that can achieve both massiveness and quantitativeness. In this approach, a pool of single-stranded DNA with a partially randomized promo...

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Detalles Bibliográficos
Autores principales: Ohuchi, Shoji, Mascher, Thorsten, Suess, Beatrix
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395800/
https://www.ncbi.nlm.nih.gov/pubmed/30816266
http://dx.doi.org/10.1038/s41598-019-39892-x