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Promoter RNA sequencing (PRSeq) for the massive and quantitative promoter analysis in vitro
Analysis of promoter strength and specificity is important for understanding and engineering gene regulation. Here, we report an in vitro promoter analysis method that can achieve both massiveness and quantitativeness. In this approach, a pool of single-stranded DNA with a partially randomized promo...
Autores principales: | Ohuchi, Shoji, Mascher, Thorsten, Suess, Beatrix |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395800/ https://www.ncbi.nlm.nih.gov/pubmed/30816266 http://dx.doi.org/10.1038/s41598-019-39892-x |
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