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Hidden in plain sight: what remains to be discovered in the eukaryotic proteome?
The first decade of genome sequencing stimulated an explosion in the characterization of unknown proteins. More recently, the pace of functional discovery has slowed, leaving around 20% of the proteins even in well-studied model organisms without informative descriptions of their biological roles. R...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395881/ https://www.ncbi.nlm.nih.gov/pubmed/30938578 http://dx.doi.org/10.1098/rsob.180241 |
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author | Wood, Valerie Lock, Antonia Harris, Midori A. Rutherford, Kim Bähler, Jürg Oliver, Stephen G. |
author_facet | Wood, Valerie Lock, Antonia Harris, Midori A. Rutherford, Kim Bähler, Jürg Oliver, Stephen G. |
author_sort | Wood, Valerie |
collection | PubMed |
description | The first decade of genome sequencing stimulated an explosion in the characterization of unknown proteins. More recently, the pace of functional discovery has slowed, leaving around 20% of the proteins even in well-studied model organisms without informative descriptions of their biological roles. Remarkably, many uncharacterized proteins are conserved from yeasts to human, suggesting that they contribute to fundamental biological processes (BP). To fully understand biological systems in health and disease, we need to account for every part of the system. Unstudied proteins thus represent a collective blind spot that limits the progress of both basic and applied biosciences. We use a simple yet powerful metric based on Gene Ontology BP terms to define characterized and uncharacterized proteins for human, budding yeast and fission yeast. We then identify a set of conserved but unstudied proteins in S. pombe, and classify them based on a combination of orthogonal attributes determined by large-scale experimental and comparative methods. Finally, we explore possible reasons why these proteins remain neglected, and propose courses of action to raise their profile and thereby reap the benefits of completing the catalogue of proteins’ biological roles. |
format | Online Article Text |
id | pubmed-6395881 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-63958812019-03-05 Hidden in plain sight: what remains to be discovered in the eukaryotic proteome? Wood, Valerie Lock, Antonia Harris, Midori A. Rutherford, Kim Bähler, Jürg Oliver, Stephen G. Open Biol Research The first decade of genome sequencing stimulated an explosion in the characterization of unknown proteins. More recently, the pace of functional discovery has slowed, leaving around 20% of the proteins even in well-studied model organisms without informative descriptions of their biological roles. Remarkably, many uncharacterized proteins are conserved from yeasts to human, suggesting that they contribute to fundamental biological processes (BP). To fully understand biological systems in health and disease, we need to account for every part of the system. Unstudied proteins thus represent a collective blind spot that limits the progress of both basic and applied biosciences. We use a simple yet powerful metric based on Gene Ontology BP terms to define characterized and uncharacterized proteins for human, budding yeast and fission yeast. We then identify a set of conserved but unstudied proteins in S. pombe, and classify them based on a combination of orthogonal attributes determined by large-scale experimental and comparative methods. Finally, we explore possible reasons why these proteins remain neglected, and propose courses of action to raise their profile and thereby reap the benefits of completing the catalogue of proteins’ biological roles. The Royal Society 2019-02-20 /pmc/articles/PMC6395881/ /pubmed/30938578 http://dx.doi.org/10.1098/rsob.180241 Text en © 2019 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Research Wood, Valerie Lock, Antonia Harris, Midori A. Rutherford, Kim Bähler, Jürg Oliver, Stephen G. Hidden in plain sight: what remains to be discovered in the eukaryotic proteome? |
title | Hidden in plain sight: what remains to be discovered in the eukaryotic proteome? |
title_full | Hidden in plain sight: what remains to be discovered in the eukaryotic proteome? |
title_fullStr | Hidden in plain sight: what remains to be discovered in the eukaryotic proteome? |
title_full_unstemmed | Hidden in plain sight: what remains to be discovered in the eukaryotic proteome? |
title_short | Hidden in plain sight: what remains to be discovered in the eukaryotic proteome? |
title_sort | hidden in plain sight: what remains to be discovered in the eukaryotic proteome? |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395881/ https://www.ncbi.nlm.nih.gov/pubmed/30938578 http://dx.doi.org/10.1098/rsob.180241 |
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