Cargando…

Microvesicles containing miR-34a induce apoptosis of proximal tubular epithelial cells and participate in renal interstitial fibrosis

Function and potential mechanism of microvesicles (MVs) containing microRNA34a in renal interstitial fibrosis were investigated. A rat model of renal interstitial fibrosis was established by unilateral ureteral ligation (UUO). Rat proximal tubular epithelial cell line (NRK-52E) was used to explore t...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Hongyan, Xu, Yuexia, Zhang, Qin, Xu, Hongfang, Xu, Yan, Ling, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396007/
https://www.ncbi.nlm.nih.gov/pubmed/30867715
http://dx.doi.org/10.3892/etm.2019.7197
_version_ 1783399184572874752
author Li, Hongyan
Xu, Yuexia
Zhang, Qin
Xu, Hongfang
Xu, Yan
Ling, Kai
author_facet Li, Hongyan
Xu, Yuexia
Zhang, Qin
Xu, Hongfang
Xu, Yan
Ling, Kai
author_sort Li, Hongyan
collection PubMed
description Function and potential mechanism of microvesicles (MVs) containing microRNA34a in renal interstitial fibrosis were investigated. A rat model of renal interstitial fibrosis was established by unilateral ureteral ligation (UUO). Rat proximal tubular epithelial cell line (NRK-52E) was used to explore the effect of MVs containing microRNA-34a on tubular epithelial cells during fibrosis, which were secreted by tubulointerstitial fibroblasts. Regardless of the UUO renal interstitial fibrosis model, or the TGF-β1-treated renal tubular epithelial cells, microRNA-34a was increased in the MVs secreted by tubulointerstitial fibroblasts. miR-34a could be transmitted through the damaged tubule basement membrane to proximal tubular epithelial cells, where it induced apoptosis of renal tubular epithelial cells by inhibiting the expression of Bcl-2, further aggravating renal interstitial fibrosis. MicroRNA-34a secreted by damaged renal interstitial fibroblasts can promote renal tubular epithelial cell apoptosis and participate in renal interstitial fibrosis by inhibiting Bcl-2.
format Online
Article
Text
id pubmed-6396007
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-63960072019-03-13 Microvesicles containing miR-34a induce apoptosis of proximal tubular epithelial cells and participate in renal interstitial fibrosis Li, Hongyan Xu, Yuexia Zhang, Qin Xu, Hongfang Xu, Yan Ling, Kai Exp Ther Med Articles Function and potential mechanism of microvesicles (MVs) containing microRNA34a in renal interstitial fibrosis were investigated. A rat model of renal interstitial fibrosis was established by unilateral ureteral ligation (UUO). Rat proximal tubular epithelial cell line (NRK-52E) was used to explore the effect of MVs containing microRNA-34a on tubular epithelial cells during fibrosis, which were secreted by tubulointerstitial fibroblasts. Regardless of the UUO renal interstitial fibrosis model, or the TGF-β1-treated renal tubular epithelial cells, microRNA-34a was increased in the MVs secreted by tubulointerstitial fibroblasts. miR-34a could be transmitted through the damaged tubule basement membrane to proximal tubular epithelial cells, where it induced apoptosis of renal tubular epithelial cells by inhibiting the expression of Bcl-2, further aggravating renal interstitial fibrosis. MicroRNA-34a secreted by damaged renal interstitial fibroblasts can promote renal tubular epithelial cell apoptosis and participate in renal interstitial fibrosis by inhibiting Bcl-2. D.A. Spandidos 2019-03 2019-01-24 /pmc/articles/PMC6396007/ /pubmed/30867715 http://dx.doi.org/10.3892/etm.2019.7197 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Li, Hongyan
Xu, Yuexia
Zhang, Qin
Xu, Hongfang
Xu, Yan
Ling, Kai
Microvesicles containing miR-34a induce apoptosis of proximal tubular epithelial cells and participate in renal interstitial fibrosis
title Microvesicles containing miR-34a induce apoptosis of proximal tubular epithelial cells and participate in renal interstitial fibrosis
title_full Microvesicles containing miR-34a induce apoptosis of proximal tubular epithelial cells and participate in renal interstitial fibrosis
title_fullStr Microvesicles containing miR-34a induce apoptosis of proximal tubular epithelial cells and participate in renal interstitial fibrosis
title_full_unstemmed Microvesicles containing miR-34a induce apoptosis of proximal tubular epithelial cells and participate in renal interstitial fibrosis
title_short Microvesicles containing miR-34a induce apoptosis of proximal tubular epithelial cells and participate in renal interstitial fibrosis
title_sort microvesicles containing mir-34a induce apoptosis of proximal tubular epithelial cells and participate in renal interstitial fibrosis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396007/
https://www.ncbi.nlm.nih.gov/pubmed/30867715
http://dx.doi.org/10.3892/etm.2019.7197
work_keys_str_mv AT lihongyan microvesiclescontainingmir34ainduceapoptosisofproximaltubularepithelialcellsandparticipateinrenalinterstitialfibrosis
AT xuyuexia microvesiclescontainingmir34ainduceapoptosisofproximaltubularepithelialcellsandparticipateinrenalinterstitialfibrosis
AT zhangqin microvesiclescontainingmir34ainduceapoptosisofproximaltubularepithelialcellsandparticipateinrenalinterstitialfibrosis
AT xuhongfang microvesiclescontainingmir34ainduceapoptosisofproximaltubularepithelialcellsandparticipateinrenalinterstitialfibrosis
AT xuyan microvesiclescontainingmir34ainduceapoptosisofproximaltubularepithelialcellsandparticipateinrenalinterstitialfibrosis
AT lingkai microvesiclescontainingmir34ainduceapoptosisofproximaltubularepithelialcellsandparticipateinrenalinterstitialfibrosis