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Substrate-Specific Inhibition Constants for Phospholipase A(2) Acting on Unique Phospholipid Substrates in Mixed Micelles and Membranes Using Lipidomics
[Image: see text] Assaying lipolytic enzymes is extremely challenging because they act on water-insoluble lipid substrates, which are normally components of micelles, vesicles, and cellular membranes. We extended a new lipidomics-based liquid chromatographic–mass spectrometric assay for phospholipas...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical
Society
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6398150/ https://www.ncbi.nlm.nih.gov/pubmed/30615445 http://dx.doi.org/10.1021/acs.jmedchem.8b01568 |