A bio-coupling approach using a dextran-binding domain to immobilize an engineered streptavidin to Sephadex for easy preparation of affinity matrix

An engineered streptavidin, SAVSBPM18 with reversible biotin binding capability, has been successfully applied to purify biotinylated and streptavidin-binding peptide (SBP) tagged proteins. To simplify the preparation for the SAVSBPM18 affinity matrix without chemical conjugation, two bio-coupling a...

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Detalles Bibliográficos
Autores principales: Wu, Sau-Ching, Wang, Chris, Chin, Jonathan, Wong, Sui-Lam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399347/
https://www.ncbi.nlm.nih.gov/pubmed/30833609
http://dx.doi.org/10.1038/s41598-019-40044-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399347/
https://www.ncbi.nlm.nih.gov/pubmed/30833609
http://dx.doi.org/10.1038/s41598-019-40044-4

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