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Light-Controlled Affinity Purification of Protein Complexes Exemplified by the Resting ZAP70 Interactome

Multiprotein complexes control the behavior of cells, such as of lymphocytes of the immune system. Methods to affinity purify protein complexes and to determine their interactome by mass spectrometry are thus widely used. One drawback of these methods is the presence of false positives. In fact, the...

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Detalles Bibliográficos
Autores principales: Hörner, Maximilian, Eble, Julian, Yousefi, O. Sascha, Schwarz, Jennifer, Warscheid, Bettina, Weber, Wilfried, Schamel, Wolfgang W. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399385/
https://www.ncbi.nlm.nih.gov/pubmed/30863395
http://dx.doi.org/10.3389/fimmu.2019.00226