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Association Between the Methylation Statuses at CpG Sites in the Promoter Region of the SLCO1B3, RNA Expression and Color Change in Blue Eggshells in Lushi Chickens

The formation mechanism underlying the blue eggshell characteristic has been discovered in birds, and SLCO1B3 is the key gene that regulates the blue eggshell color. Insertion of an endogenous retrovirus, EAV-HP, in the SLCO1B3 5′ flanking region promotes SLCO1B3 expression in the chicken shell glan...

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Autores principales: Li, Zhuanjian, Ren, Tuanhui, Li, Wenya, Zhou, Yu, Han, Ruili, Li, Hong, Jiang, Ruirui, Yan, Fengbin, Sun, Guirong, Liu, Xiaojun, Tian, Yadong, Kang, Xiangtao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399514/
https://www.ncbi.nlm.nih.gov/pubmed/30863430
http://dx.doi.org/10.3389/fgene.2019.00161
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author Li, Zhuanjian
Ren, Tuanhui
Li, Wenya
Zhou, Yu
Han, Ruili
Li, Hong
Jiang, Ruirui
Yan, Fengbin
Sun, Guirong
Liu, Xiaojun
Tian, Yadong
Kang, Xiangtao
author_facet Li, Zhuanjian
Ren, Tuanhui
Li, Wenya
Zhou, Yu
Han, Ruili
Li, Hong
Jiang, Ruirui
Yan, Fengbin
Sun, Guirong
Liu, Xiaojun
Tian, Yadong
Kang, Xiangtao
author_sort Li, Zhuanjian
collection PubMed
description The formation mechanism underlying the blue eggshell characteristic has been discovered in birds, and SLCO1B3 is the key gene that regulates the blue eggshell color. Insertion of an endogenous retrovirus, EAV-HP, in the SLCO1B3 5′ flanking region promotes SLCO1B3 expression in the chicken shell gland, and this expression causes bile salts to enter the shell gland, where biliverdin is secreted into the eggshell, forming a blue shell. However, at different laying stages of the same group of chickens, the color of the eggshell can vary widely, and the molecular mechanism underlying the eggshell color change remains unknown. Therefore, to reveal the molecular mechanism of the blue eggshell color variations, we analyzed the change in the eggshell color during the laying period. The results indicated that the eggshell color in Lushi chickens can be divided into three stages: 20–25 weeks for dark blue, 26–45 weeks for medium blue, and 46–60 weeks for light blue. We further investigated the expression and methylation levels of the SLCO1B3 gene at eight different weeks, finding that the relative expression of SLCO1B3 was significantly higher at 25 and 30 weeks than at other laying weeks. Furthermore, the overall methylation rate of the SLCO1B3 gene in Lushi chickens increased gradually with increasing weeks of egg production, as shown by bisulfite sequencing PCR. Pearson correlation analysis showed that methylation of the promoter region of SLCO1B3 was significantly negatively correlated with both SLCO1B3 expression in the shell gland tissue and eggshell color. In addition, we predicted that CpG5 and CpG8 may be key sites for regulating SLCO1B3 gene transcription. Our findings show that as the level of methylation increases, methylation of the CpG5 and CpG8 sites hinders the binding of transcription factors to the promoter, reducing SLCO1B3 expression during the late period and resulting in a lighter eggshell color.
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spelling pubmed-63995142019-03-12 Association Between the Methylation Statuses at CpG Sites in the Promoter Region of the SLCO1B3, RNA Expression and Color Change in Blue Eggshells in Lushi Chickens Li, Zhuanjian Ren, Tuanhui Li, Wenya Zhou, Yu Han, Ruili Li, Hong Jiang, Ruirui Yan, Fengbin Sun, Guirong Liu, Xiaojun Tian, Yadong Kang, Xiangtao Front Genet Genetics The formation mechanism underlying the blue eggshell characteristic has been discovered in birds, and SLCO1B3 is the key gene that regulates the blue eggshell color. Insertion of an endogenous retrovirus, EAV-HP, in the SLCO1B3 5′ flanking region promotes SLCO1B3 expression in the chicken shell gland, and this expression causes bile salts to enter the shell gland, where biliverdin is secreted into the eggshell, forming a blue shell. However, at different laying stages of the same group of chickens, the color of the eggshell can vary widely, and the molecular mechanism underlying the eggshell color change remains unknown. Therefore, to reveal the molecular mechanism of the blue eggshell color variations, we analyzed the change in the eggshell color during the laying period. The results indicated that the eggshell color in Lushi chickens can be divided into three stages: 20–25 weeks for dark blue, 26–45 weeks for medium blue, and 46–60 weeks for light blue. We further investigated the expression and methylation levels of the SLCO1B3 gene at eight different weeks, finding that the relative expression of SLCO1B3 was significantly higher at 25 and 30 weeks than at other laying weeks. Furthermore, the overall methylation rate of the SLCO1B3 gene in Lushi chickens increased gradually with increasing weeks of egg production, as shown by bisulfite sequencing PCR. Pearson correlation analysis showed that methylation of the promoter region of SLCO1B3 was significantly negatively correlated with both SLCO1B3 expression in the shell gland tissue and eggshell color. In addition, we predicted that CpG5 and CpG8 may be key sites for regulating SLCO1B3 gene transcription. Our findings show that as the level of methylation increases, methylation of the CpG5 and CpG8 sites hinders the binding of transcription factors to the promoter, reducing SLCO1B3 expression during the late period and resulting in a lighter eggshell color. Frontiers Media S.A. 2019-02-26 /pmc/articles/PMC6399514/ /pubmed/30863430 http://dx.doi.org/10.3389/fgene.2019.00161 Text en Copyright © 2019 Li, Ren, Li, Zhou, Han, Li, Jiang, Yan, Sun, Liu, Tian and Kang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Li, Zhuanjian
Ren, Tuanhui
Li, Wenya
Zhou, Yu
Han, Ruili
Li, Hong
Jiang, Ruirui
Yan, Fengbin
Sun, Guirong
Liu, Xiaojun
Tian, Yadong
Kang, Xiangtao
Association Between the Methylation Statuses at CpG Sites in the Promoter Region of the SLCO1B3, RNA Expression and Color Change in Blue Eggshells in Lushi Chickens
title Association Between the Methylation Statuses at CpG Sites in the Promoter Region of the SLCO1B3, RNA Expression and Color Change in Blue Eggshells in Lushi Chickens
title_full Association Between the Methylation Statuses at CpG Sites in the Promoter Region of the SLCO1B3, RNA Expression and Color Change in Blue Eggshells in Lushi Chickens
title_fullStr Association Between the Methylation Statuses at CpG Sites in the Promoter Region of the SLCO1B3, RNA Expression and Color Change in Blue Eggshells in Lushi Chickens
title_full_unstemmed Association Between the Methylation Statuses at CpG Sites in the Promoter Region of the SLCO1B3, RNA Expression and Color Change in Blue Eggshells in Lushi Chickens
title_short Association Between the Methylation Statuses at CpG Sites in the Promoter Region of the SLCO1B3, RNA Expression and Color Change in Blue Eggshells in Lushi Chickens
title_sort association between the methylation statuses at cpg sites in the promoter region of the slco1b3, rna expression and color change in blue eggshells in lushi chickens
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399514/
https://www.ncbi.nlm.nih.gov/pubmed/30863430
http://dx.doi.org/10.3389/fgene.2019.00161
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