Impact of Polymerase Fidelity on Background Error Rates in Next-Generation Sequencing with Unique Molecular Identifiers/Barcodes

Liquid biopsy and detection of tumor-associated mutations in cell-free circulating DNA often requires the ability to identify single nucleotide variants at allele frequencies below 0.1%. Standard sequencing protocols cannot achieve this level of sensitivity due to background noise from DNA damage an...

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Detalles Bibliográficos
Autores principales: Filges, Stefan, Yamada, Emiko, Ståhlberg, Anders, Godfrey, Tony E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401092/
https://www.ncbi.nlm.nih.gov/pubmed/30837525
http://dx.doi.org/10.1038/s41598-019-39762-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401092/
https://www.ncbi.nlm.nih.gov/pubmed/30837525
http://dx.doi.org/10.1038/s41598-019-39762-6

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