Evaluation of detection probabilities at the water-filtering and initial PCR steps in environmental DNA metabarcoding using a multispecies site occupancy model

Environmental DNA (eDNA) metabarcoding is a recently developed method to assess biodiversity based on a high-throughput parallel DNA sequencing applied to DNA present in the ecosystem. Although eDNA metabarcoding enables a rapid assessment of biodiversity, it is prone to species detection errors tha...

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Detalles Bibliográficos
Autores principales: Doi, Hideyuki, Fukaya, Keiichi, Oka, Shin-ichiro, Sato, Keiichi, Kondoh, Michio, Miya, Masaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401178/
https://www.ncbi.nlm.nih.gov/pubmed/30837589
http://dx.doi.org/10.1038/s41598-019-40233-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401178/
https://www.ncbi.nlm.nih.gov/pubmed/30837589
http://dx.doi.org/10.1038/s41598-019-40233-1

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