An Improved Transformation System for Cowpea (Vigna unguiculata L. Walp) via Sonication and a Kanamycin-Geneticin Selection Regime

An improved cowpea transformation method utilizing Agrobacterium-mediated gene delivery to explants derived from the cotyledonary nodes of imbibed cowpea seed is described. The explants were regenerated following a sonication procedure and a stringent selection comprising alternating regimes of kana...

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Detalles Bibliográficos
Autores principales: Bett, Bosibori, Gollasch, Stephanie, Moore, Andy, Harding, Robert, Higgins, Thomas J. V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401653/
https://www.ncbi.nlm.nih.gov/pubmed/30873198
http://dx.doi.org/10.3389/fpls.2019.00219

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401653/
https://www.ncbi.nlm.nih.gov/pubmed/30873198
http://dx.doi.org/10.3389/fpls.2019.00219

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