An Improved Transformation System for Cowpea (Vigna unguiculata L. Walp) via Sonication and a Kanamycin-Geneticin Selection Regime

An improved cowpea transformation method utilizing Agrobacterium-mediated gene delivery to explants derived from the cotyledonary nodes of imbibed cowpea seed is described. The explants were regenerated following a sonication procedure and a stringent selection comprising alternating regimes of kana...

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Autores principales: Bett, Bosibori, Gollasch, Stephanie, Moore, Andy, Harding, Robert, Higgins, Thomas J. V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401653/
https://www.ncbi.nlm.nih.gov/pubmed/30873198
http://dx.doi.org/10.3389/fpls.2019.00219
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author Bett, Bosibori
Gollasch, Stephanie
Moore, Andy
Harding, Robert
Higgins, Thomas J. V.
author_facet Bett, Bosibori
Gollasch, Stephanie
Moore, Andy
Harding, Robert
Higgins, Thomas J. V.
author_sort Bett, Bosibori
collection PubMed
description An improved cowpea transformation method utilizing Agrobacterium-mediated gene delivery to explants derived from the cotyledonary nodes of imbibed cowpea seed is described. The explants were regenerated following a sonication procedure and a stringent selection comprising alternating regimes of kanamycin and geneticin. The method was reproducible and led to the recovery of independent fertile transgenic plants in the greenhouse at a level of about one per cent of starting explants. A transgene encoding an insecticidal protein from Bacillus thuringiensis was used to demonstrate the efficacy of the system.
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spelling pubmed-64016532019-03-14 An Improved Transformation System for Cowpea (Vigna unguiculata L. Walp) via Sonication and a Kanamycin-Geneticin Selection Regime Bett, Bosibori Gollasch, Stephanie Moore, Andy Harding, Robert Higgins, Thomas J. V. Front Plant Sci Plant Science An improved cowpea transformation method utilizing Agrobacterium-mediated gene delivery to explants derived from the cotyledonary nodes of imbibed cowpea seed is described. The explants were regenerated following a sonication procedure and a stringent selection comprising alternating regimes of kanamycin and geneticin. The method was reproducible and led to the recovery of independent fertile transgenic plants in the greenhouse at a level of about one per cent of starting explants. A transgene encoding an insecticidal protein from Bacillus thuringiensis was used to demonstrate the efficacy of the system. Frontiers Media S.A. 2019-02-26 /pmc/articles/PMC6401653/ /pubmed/30873198 http://dx.doi.org/10.3389/fpls.2019.00219 Text en Copyright © 2019 Bett, Gollasch, Moore, Harding and Higgins. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Bett, Bosibori
Gollasch, Stephanie
Moore, Andy
Harding, Robert
Higgins, Thomas J. V.
An Improved Transformation System for Cowpea (Vigna unguiculata L. Walp) via Sonication and a Kanamycin-Geneticin Selection Regime
title An Improved Transformation System for Cowpea (Vigna unguiculata L. Walp) via Sonication and a Kanamycin-Geneticin Selection Regime
title_full An Improved Transformation System for Cowpea (Vigna unguiculata L. Walp) via Sonication and a Kanamycin-Geneticin Selection Regime
title_fullStr An Improved Transformation System for Cowpea (Vigna unguiculata L. Walp) via Sonication and a Kanamycin-Geneticin Selection Regime
title_full_unstemmed An Improved Transformation System for Cowpea (Vigna unguiculata L. Walp) via Sonication and a Kanamycin-Geneticin Selection Regime
title_short An Improved Transformation System for Cowpea (Vigna unguiculata L. Walp) via Sonication and a Kanamycin-Geneticin Selection Regime
title_sort improved transformation system for cowpea (vigna unguiculata l. walp) via sonication and a kanamycin-geneticin selection regime
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401653/
https://www.ncbi.nlm.nih.gov/pubmed/30873198
http://dx.doi.org/10.3389/fpls.2019.00219
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