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Challenging the proposed causes of the PCR plateau phase

Despite the wide-spread use of the polymerase chain reaction (PCR) in various life-science applications, the causes of arrested amplicon generation in late cycles have not been confidently identified. This so-called plateau phase has been attributed to depletion or thermal break-down of primers or n...

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Detalles Bibliográficos
Autores principales: Jansson, Linda, Hedman, Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403077/
https://www.ncbi.nlm.nih.gov/pubmed/30886826
http://dx.doi.org/10.1016/j.bdq.2019.100082