PCR effects of melting temperature adjustment of individual primers in degenerate primer pools

Deep sequencing of small subunit ribosomal RNA (SSU rRNA) gene amplicons continues to be the most common approach for characterization of complex microbial communities. PCR amplifications of conserved regions of SSU rRNA genes often employ degenerate pools of primers to enable targeting of a broad s...

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Detalles Bibliográficos
Autores principales: Naqib, Ankur, Jeon, Trisha, Kunstman, Kevin, Wang, Weihua, Shen, Yiding, Sweeney, Dagmar, Hyde, Marieta, Green, Stefan J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2019
Materias:
Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404654/
https://www.ncbi.nlm.nih.gov/pubmed/30863685
http://dx.doi.org/10.7717/peerj.6570

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404654/
https://www.ncbi.nlm.nih.gov/pubmed/30863685
http://dx.doi.org/10.7717/peerj.6570

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