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Enhanced in vivo-imaging in medaka by optimized anaesthesia, fluorescent protein selection and removal of pigmentation

Fish are ideally suited for in vivo-imaging due to their transparency at early stages combined with a large genetic toolbox. Key challenges to further advance imaging are fluorophore selection, immobilization of the specimen and approaches to eliminate pigmentation. We addressed all three and identi...

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Detalles Bibliográficos
Autores principales: Lischik, Colin Q., Adelmann, Leonie, Wittbrodt, Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405165/
https://www.ncbi.nlm.nih.gov/pubmed/30845151
http://dx.doi.org/10.1371/journal.pone.0212956