Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure

Morphological and molecular characteristics determine the function of biological tissues. Attempts to combine immunofluorescence and electron microscopy invariably compromise the quality of the ultrastructure of tissue sections. We developed NATIVE, a correlated light and electron microscopy approac...

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Detalles Bibliográficos
Autores principales: Fang, Tao, Lu, Xiaotang, Berger, Daniel, Gmeiner, Christina, Cho, Julia, Schalek, Richard, Ploegh, Hidde, Lichtman, Jeff
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405223/
https://www.ncbi.nlm.nih.gov/pubmed/30397326
http://dx.doi.org/10.1038/s41592-018-0177-x
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author Fang, Tao
Lu, Xiaotang
Berger, Daniel
Gmeiner, Christina
Cho, Julia
Schalek, Richard
Ploegh, Hidde
Lichtman, Jeff
author_facet Fang, Tao
Lu, Xiaotang
Berger, Daniel
Gmeiner, Christina
Cho, Julia
Schalek, Richard
Ploegh, Hidde
Lichtman, Jeff
author_sort Fang, Tao
collection PubMed
description Morphological and molecular characteristics determine the function of biological tissues. Attempts to combine immunofluorescence and electron microscopy invariably compromise the quality of the ultrastructure of tissue sections. We developed NATIVE, a correlated light and electron microscopy approach that preserves ultrastructure while showing the locations of multiple molecular moieties even deep within tissues. This technique allowed the large-scale 3D reconstruction of a volume of mouse hippocampal CA3 tissue at nanometer resolution.
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spelling pubmed-64052232019-05-05 Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure Fang, Tao Lu, Xiaotang Berger, Daniel Gmeiner, Christina Cho, Julia Schalek, Richard Ploegh, Hidde Lichtman, Jeff Nat Methods Article Morphological and molecular characteristics determine the function of biological tissues. Attempts to combine immunofluorescence and electron microscopy invariably compromise the quality of the ultrastructure of tissue sections. We developed NATIVE, a correlated light and electron microscopy approach that preserves ultrastructure while showing the locations of multiple molecular moieties even deep within tissues. This technique allowed the large-scale 3D reconstruction of a volume of mouse hippocampal CA3 tissue at nanometer resolution. 2018-11-05 2018-12 /pmc/articles/PMC6405223/ /pubmed/30397326 http://dx.doi.org/10.1038/s41592-018-0177-x Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Fang, Tao
Lu, Xiaotang
Berger, Daniel
Gmeiner, Christina
Cho, Julia
Schalek, Richard
Ploegh, Hidde
Lichtman, Jeff
Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure
title Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure
title_full Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure
title_fullStr Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure
title_full_unstemmed Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure
title_short Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure
title_sort nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405223/
https://www.ncbi.nlm.nih.gov/pubmed/30397326
http://dx.doi.org/10.1038/s41592-018-0177-x
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