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Imaging inflammation using an activated macrophage probe with Slc18b1 as the activation-selective gating target
Activated macrophages have the potential to be ideal targets for imaging inflammation. However, probe selectivity over non-activated macrophages and probe delivery to target tissue have been challenging. Here, we report a small molecule probe specific for activated macrophages, called CDg16, and dem...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405920/ https://www.ncbi.nlm.nih.gov/pubmed/30846702 http://dx.doi.org/10.1038/s41467-019-08990-9 |
| _version_ | 1783401187163242496 |
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| author | Park, Sung-Jin Kim, Beomsue Choi, Sejong Balasubramaniam, Sivaraman Lee, Sung-Chan Lee, Jung Yeol Kim, Heon Seok Kim, Jun-Young Kim, Jong-Jin Lee, Yong-An Kang, Nam-Young Kim, Jin-Soo Chang, Young-Tae |
| author_facet | Park, Sung-Jin Kim, Beomsue Choi, Sejong Balasubramaniam, Sivaraman Lee, Sung-Chan Lee, Jung Yeol Kim, Heon Seok Kim, Jun-Young Kim, Jong-Jin Lee, Yong-An Kang, Nam-Young Kim, Jin-Soo Chang, Young-Tae |
| author_sort | Park, Sung-Jin |
| collection | PubMed |
| description | Activated macrophages have the potential to be ideal targets for imaging inflammation. However, probe selectivity over non-activated macrophages and probe delivery to target tissue have been challenging. Here, we report a small molecule probe specific for activated macrophages, called CDg16, and demonstrate its application to visualizing inflammatory atherosclerotic plaques in vivo. Through a systematic transporter screen using a CRISPR activation library, we identify the orphan transporter Slc18b1/SLC18B1 as the gating target of CDg16. |
| format | Online Article Text |
| id | pubmed-6405920 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2019 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-64059202019-03-11 Imaging inflammation using an activated macrophage probe with Slc18b1 as the activation-selective gating target Park, Sung-Jin Kim, Beomsue Choi, Sejong Balasubramaniam, Sivaraman Lee, Sung-Chan Lee, Jung Yeol Kim, Heon Seok Kim, Jun-Young Kim, Jong-Jin Lee, Yong-An Kang, Nam-Young Kim, Jin-Soo Chang, Young-Tae Nat Commun Article Activated macrophages have the potential to be ideal targets for imaging inflammation. However, probe selectivity over non-activated macrophages and probe delivery to target tissue have been challenging. Here, we report a small molecule probe specific for activated macrophages, called CDg16, and demonstrate its application to visualizing inflammatory atherosclerotic plaques in vivo. Through a systematic transporter screen using a CRISPR activation library, we identify the orphan transporter Slc18b1/SLC18B1 as the gating target of CDg16. Nature Publishing Group UK 2019-03-07 /pmc/articles/PMC6405920/ /pubmed/30846702 http://dx.doi.org/10.1038/s41467-019-08990-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| spellingShingle | Article Park, Sung-Jin Kim, Beomsue Choi, Sejong Balasubramaniam, Sivaraman Lee, Sung-Chan Lee, Jung Yeol Kim, Heon Seok Kim, Jun-Young Kim, Jong-Jin Lee, Yong-An Kang, Nam-Young Kim, Jin-Soo Chang, Young-Tae Imaging inflammation using an activated macrophage probe with Slc18b1 as the activation-selective gating target |
| title | Imaging inflammation using an activated macrophage probe with Slc18b1 as the activation-selective gating target |
| title_full | Imaging inflammation using an activated macrophage probe with Slc18b1 as the activation-selective gating target |
| title_fullStr | Imaging inflammation using an activated macrophage probe with Slc18b1 as the activation-selective gating target |
| title_full_unstemmed | Imaging inflammation using an activated macrophage probe with Slc18b1 as the activation-selective gating target |
| title_short | Imaging inflammation using an activated macrophage probe with Slc18b1 as the activation-selective gating target |
| title_sort | imaging inflammation using an activated macrophage probe with slc18b1 as the activation-selective gating target |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6405920/ https://www.ncbi.nlm.nih.gov/pubmed/30846702 http://dx.doi.org/10.1038/s41467-019-08990-9 |
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