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Plasma-Treated Air and Water—Assessment of Synergistic Antimicrobial Effects for Sanitation of Food Processing Surfaces and Environment
The synergistic antimicrobial effects of plasma-processed air (PPA) and plasma-treated water (PTW), which are indirectly generated by a microwave-induced non-atmospheric pressure plasma, were investigated with the aid of proliferation assays. For this purpose, microorganisms (Listeria monocytogenes,...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406376/ https://www.ncbi.nlm.nih.gov/pubmed/30717375 http://dx.doi.org/10.3390/foods8020055 |
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author | Schnabel, Uta Handorf, Oliver Yarova, Kateryna Zessin, Björn Zechlin, Susann Sydow, Diana Zellmer, Elke Stachowiak, Jörg Andrasch, Mathias Below, Harald Ehlbeck, Jörg |
author_facet | Schnabel, Uta Handorf, Oliver Yarova, Kateryna Zessin, Björn Zechlin, Susann Sydow, Diana Zellmer, Elke Stachowiak, Jörg Andrasch, Mathias Below, Harald Ehlbeck, Jörg |
author_sort | Schnabel, Uta |
collection | PubMed |
description | The synergistic antimicrobial effects of plasma-processed air (PPA) and plasma-treated water (PTW), which are indirectly generated by a microwave-induced non-atmospheric pressure plasma, were investigated with the aid of proliferation assays. For this purpose, microorganisms (Listeria monocytogenes, Escherichia coli, Pectobacterium carotovorum, sporulated Bacillus atrophaeus) were cultivated as monocultures on specimens with polymeric surface structures. Both the distinct and synergistic antimicrobial potential of PPA and PTW were governed by the plasma-on time (5–50 s) and the treatment time of the specimens with PPA/PTW (1–5 min). In single PTW treatment of the bacteria, an elevation of the reduction factor with increasing treatment time could be observed (e.g., reduction factor of 2.4 to 3.0 for P. carotovorum). In comparison, the combination of PTW and subsequent PPA treatment leads to synergistic effects that are clearly not induced by longer treatment times. These findings have been valid for all bacteria (L. monocytogenes > P. carotovorum = E. coli). Controversially, the effect is reversed for endospores of B. atrophaeus. With pure PPA treatment, a strong inactivation at 50 s plasma-on time is detectable, whereas single PTW treatment shows no effect even with increasing treatment parameters. The use of synergistic effects of PTW for cleaning and PPA for drying shows a clear alternative for currently used sanitation methods in production plants. Highlights: Non-thermal atmospheric pressure microwave plasma source used indirect in two different modes—gaseous and liquid; Measurement of short and long-living nitrite and nitrate in corrosive gas PPA (plasma-processed air) and complex liquid PTW (plasma-treated water); Application of PTW and PPA in single and combined use for biological decontamination of different microorganisms. |
format | Online Article Text |
id | pubmed-6406376 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64063762019-03-19 Plasma-Treated Air and Water—Assessment of Synergistic Antimicrobial Effects for Sanitation of Food Processing Surfaces and Environment Schnabel, Uta Handorf, Oliver Yarova, Kateryna Zessin, Björn Zechlin, Susann Sydow, Diana Zellmer, Elke Stachowiak, Jörg Andrasch, Mathias Below, Harald Ehlbeck, Jörg Foods Article The synergistic antimicrobial effects of plasma-processed air (PPA) and plasma-treated water (PTW), which are indirectly generated by a microwave-induced non-atmospheric pressure plasma, were investigated with the aid of proliferation assays. For this purpose, microorganisms (Listeria monocytogenes, Escherichia coli, Pectobacterium carotovorum, sporulated Bacillus atrophaeus) were cultivated as monocultures on specimens with polymeric surface structures. Both the distinct and synergistic antimicrobial potential of PPA and PTW were governed by the plasma-on time (5–50 s) and the treatment time of the specimens with PPA/PTW (1–5 min). In single PTW treatment of the bacteria, an elevation of the reduction factor with increasing treatment time could be observed (e.g., reduction factor of 2.4 to 3.0 for P. carotovorum). In comparison, the combination of PTW and subsequent PPA treatment leads to synergistic effects that are clearly not induced by longer treatment times. These findings have been valid for all bacteria (L. monocytogenes > P. carotovorum = E. coli). Controversially, the effect is reversed for endospores of B. atrophaeus. With pure PPA treatment, a strong inactivation at 50 s plasma-on time is detectable, whereas single PTW treatment shows no effect even with increasing treatment parameters. The use of synergistic effects of PTW for cleaning and PPA for drying shows a clear alternative for currently used sanitation methods in production plants. Highlights: Non-thermal atmospheric pressure microwave plasma source used indirect in two different modes—gaseous and liquid; Measurement of short and long-living nitrite and nitrate in corrosive gas PPA (plasma-processed air) and complex liquid PTW (plasma-treated water); Application of PTW and PPA in single and combined use for biological decontamination of different microorganisms. MDPI 2019-02-02 /pmc/articles/PMC6406376/ /pubmed/30717375 http://dx.doi.org/10.3390/foods8020055 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Schnabel, Uta Handorf, Oliver Yarova, Kateryna Zessin, Björn Zechlin, Susann Sydow, Diana Zellmer, Elke Stachowiak, Jörg Andrasch, Mathias Below, Harald Ehlbeck, Jörg Plasma-Treated Air and Water—Assessment of Synergistic Antimicrobial Effects for Sanitation of Food Processing Surfaces and Environment |
title | Plasma-Treated Air and Water—Assessment of Synergistic Antimicrobial Effects for Sanitation of Food Processing Surfaces and Environment |
title_full | Plasma-Treated Air and Water—Assessment of Synergistic Antimicrobial Effects for Sanitation of Food Processing Surfaces and Environment |
title_fullStr | Plasma-Treated Air and Water—Assessment of Synergistic Antimicrobial Effects for Sanitation of Food Processing Surfaces and Environment |
title_full_unstemmed | Plasma-Treated Air and Water—Assessment of Synergistic Antimicrobial Effects for Sanitation of Food Processing Surfaces and Environment |
title_short | Plasma-Treated Air and Water—Assessment of Synergistic Antimicrobial Effects for Sanitation of Food Processing Surfaces and Environment |
title_sort | plasma-treated air and water—assessment of synergistic antimicrobial effects for sanitation of food processing surfaces and environment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406376/ https://www.ncbi.nlm.nih.gov/pubmed/30717375 http://dx.doi.org/10.3390/foods8020055 |
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