CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine miR-17-92 Cluster

Successful RNAi applications depend on strategies allowing stable and persistent expression of minimal gene silencing triggers without perturbing endogenous gene expression. In this study, we proposed an endogenous microRNA (miRNA) cluster as a novel integration site for small hairpin RNAs (shRNAs)....

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Detalles Bibliográficos
Autores principales: Lu, Chao, Pang, Daxin, Li, Mengjing, Yuan, Hongming, Yu, Tingting, Huang, Peixuan, Li, Jianing, Chen, Xue, Jiao, Huping, Xie, Zicong, Ouyang, Hongsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406430/
https://www.ncbi.nlm.nih.gov/pubmed/30717310
http://dx.doi.org/10.3390/cells8020113

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406430/
https://www.ncbi.nlm.nih.gov/pubmed/30717310
http://dx.doi.org/10.3390/cells8020113

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