Cargando…
Immunomagnetic-Enriched Subpopulations of Melanoma Circulating Tumour Cells (CTCs) Exhibit Distinct Transcriptome Profiles
Cutaneous melanoma circulating tumour cells (CTCs) are phenotypically and molecularly heterogeneous. We profiled the gene expression of CTC subpopulations immunomagnetic-captured by targeting either the melanoma-associated marker, MCSP, or the melanoma-initiating marker, ABCB5. Firstly, the expressi...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406574/ https://www.ncbi.nlm.nih.gov/pubmed/30769764 http://dx.doi.org/10.3390/cancers11020157 |
_version_ | 1783401341951934464 |
---|---|
author | Aya-Bonilla, Carlos Gray, Elin S. Manikandan, Jayapal Freeman, James B. Zaenker, Pauline Reid, Anna L. Khattak, Muhammad A. Frank, Markus H. Millward, Michael Ziman, Mel |
author_facet | Aya-Bonilla, Carlos Gray, Elin S. Manikandan, Jayapal Freeman, James B. Zaenker, Pauline Reid, Anna L. Khattak, Muhammad A. Frank, Markus H. Millward, Michael Ziman, Mel |
author_sort | Aya-Bonilla, Carlos |
collection | PubMed |
description | Cutaneous melanoma circulating tumour cells (CTCs) are phenotypically and molecularly heterogeneous. We profiled the gene expression of CTC subpopulations immunomagnetic-captured by targeting either the melanoma-associated marker, MCSP, or the melanoma-initiating marker, ABCB5. Firstly, the expression of a subset of melanoma genes was investigated by RT-PCR in MCSP-enriched and ABCB5-enriched CTCs isolated from a total of 59 blood draws from 39 melanoma cases. Of these, 6 MCSP- and 6 ABCB5-enriched CTC fractions were further analysed using a genome-wide gene expression microarray. The transcriptional programs of both CTC subtypes included cell survival maintenance, cell proliferation, and migration pathways. ABCB5-enriched CTCs were specifically characterised by up-regulation of genes involved in epithelial to mesenchymal transition (EMT), suggesting an invasive phenotype. These findings underscore the presence of at least two distinct melanoma CTC subpopulations with distinct transcriptional programs, which may have distinct roles in disease progression and response to therapy. |
format | Online Article Text |
id | pubmed-6406574 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64065742019-03-21 Immunomagnetic-Enriched Subpopulations of Melanoma Circulating Tumour Cells (CTCs) Exhibit Distinct Transcriptome Profiles Aya-Bonilla, Carlos Gray, Elin S. Manikandan, Jayapal Freeman, James B. Zaenker, Pauline Reid, Anna L. Khattak, Muhammad A. Frank, Markus H. Millward, Michael Ziman, Mel Cancers (Basel) Article Cutaneous melanoma circulating tumour cells (CTCs) are phenotypically and molecularly heterogeneous. We profiled the gene expression of CTC subpopulations immunomagnetic-captured by targeting either the melanoma-associated marker, MCSP, or the melanoma-initiating marker, ABCB5. Firstly, the expression of a subset of melanoma genes was investigated by RT-PCR in MCSP-enriched and ABCB5-enriched CTCs isolated from a total of 59 blood draws from 39 melanoma cases. Of these, 6 MCSP- and 6 ABCB5-enriched CTC fractions were further analysed using a genome-wide gene expression microarray. The transcriptional programs of both CTC subtypes included cell survival maintenance, cell proliferation, and migration pathways. ABCB5-enriched CTCs were specifically characterised by up-regulation of genes involved in epithelial to mesenchymal transition (EMT), suggesting an invasive phenotype. These findings underscore the presence of at least two distinct melanoma CTC subpopulations with distinct transcriptional programs, which may have distinct roles in disease progression and response to therapy. MDPI 2019-01-30 /pmc/articles/PMC6406574/ /pubmed/30769764 http://dx.doi.org/10.3390/cancers11020157 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Aya-Bonilla, Carlos Gray, Elin S. Manikandan, Jayapal Freeman, James B. Zaenker, Pauline Reid, Anna L. Khattak, Muhammad A. Frank, Markus H. Millward, Michael Ziman, Mel Immunomagnetic-Enriched Subpopulations of Melanoma Circulating Tumour Cells (CTCs) Exhibit Distinct Transcriptome Profiles |
title | Immunomagnetic-Enriched Subpopulations of Melanoma Circulating Tumour Cells (CTCs) Exhibit Distinct Transcriptome Profiles |
title_full | Immunomagnetic-Enriched Subpopulations of Melanoma Circulating Tumour Cells (CTCs) Exhibit Distinct Transcriptome Profiles |
title_fullStr | Immunomagnetic-Enriched Subpopulations of Melanoma Circulating Tumour Cells (CTCs) Exhibit Distinct Transcriptome Profiles |
title_full_unstemmed | Immunomagnetic-Enriched Subpopulations of Melanoma Circulating Tumour Cells (CTCs) Exhibit Distinct Transcriptome Profiles |
title_short | Immunomagnetic-Enriched Subpopulations of Melanoma Circulating Tumour Cells (CTCs) Exhibit Distinct Transcriptome Profiles |
title_sort | immunomagnetic-enriched subpopulations of melanoma circulating tumour cells (ctcs) exhibit distinct transcriptome profiles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406574/ https://www.ncbi.nlm.nih.gov/pubmed/30769764 http://dx.doi.org/10.3390/cancers11020157 |
work_keys_str_mv | AT ayabonillacarlos immunomagneticenrichedsubpopulationsofmelanomacirculatingtumourcellsctcsexhibitdistincttranscriptomeprofiles AT grayelins immunomagneticenrichedsubpopulationsofmelanomacirculatingtumourcellsctcsexhibitdistincttranscriptomeprofiles AT manikandanjayapal immunomagneticenrichedsubpopulationsofmelanomacirculatingtumourcellsctcsexhibitdistincttranscriptomeprofiles AT freemanjamesb immunomagneticenrichedsubpopulationsofmelanomacirculatingtumourcellsctcsexhibitdistincttranscriptomeprofiles AT zaenkerpauline immunomagneticenrichedsubpopulationsofmelanomacirculatingtumourcellsctcsexhibitdistincttranscriptomeprofiles AT reidannal immunomagneticenrichedsubpopulationsofmelanomacirculatingtumourcellsctcsexhibitdistincttranscriptomeprofiles AT khattakmuhammada immunomagneticenrichedsubpopulationsofmelanomacirculatingtumourcellsctcsexhibitdistincttranscriptomeprofiles AT frankmarkush immunomagneticenrichedsubpopulationsofmelanomacirculatingtumourcellsctcsexhibitdistincttranscriptomeprofiles AT millwardmichael immunomagneticenrichedsubpopulationsofmelanomacirculatingtumourcellsctcsexhibitdistincttranscriptomeprofiles AT zimanmel immunomagneticenrichedsubpopulationsofmelanomacirculatingtumourcellsctcsexhibitdistincttranscriptomeprofiles |