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Antioxidant Characterization and Biological Effects of Grape Pomace Extracts Supplementation in Caenorhabditis elegans

The aim of this work was to evaluate the biological activity of four grape pomace (GP) extracts that are rich in polyphenols using C. elegans as an in vivo model. Different concentrations of the GP extracts were assessed for their effects on the resistance of C. elegans against thermally induced oxi...

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Autores principales: Ayuda-Durán, Begoña, González-Manzano, Susana, Gil-Sánchez, Irene, Moreno-Arribas, M. Victoria, Bartolomé, Begoña, Sanz-Buenhombre, Marisa, Guadarrama, Alberto, Santos-Buelga, Celestino, González-Paramás, Ana M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406641/
https://www.ncbi.nlm.nih.gov/pubmed/30781355
http://dx.doi.org/10.3390/foods8020075
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author Ayuda-Durán, Begoña
González-Manzano, Susana
Gil-Sánchez, Irene
Moreno-Arribas, M. Victoria
Bartolomé, Begoña
Sanz-Buenhombre, Marisa
Guadarrama, Alberto
Santos-Buelga, Celestino
González-Paramás, Ana M.
author_facet Ayuda-Durán, Begoña
González-Manzano, Susana
Gil-Sánchez, Irene
Moreno-Arribas, M. Victoria
Bartolomé, Begoña
Sanz-Buenhombre, Marisa
Guadarrama, Alberto
Santos-Buelga, Celestino
González-Paramás, Ana M.
author_sort Ayuda-Durán, Begoña
collection PubMed
description The aim of this work was to evaluate the biological activity of four grape pomace (GP) extracts that are rich in polyphenols using C. elegans as an in vivo model. Different concentrations of the GP extracts were assessed for their effects on the resistance of C. elegans against thermally induced oxidative stress, accumulation of reactive oxygen species (ROS), and lifespan. The cultivation of C. elegans with relatively low concentrations of GP extracts increased their resistance against thermal stress and prolonged their lifespan, while high levels displayed detrimental effects. In the studied extracts, maximum protection was observed for levels of polyphenols around 7 to 9 µg gallic acid equivalents per cultivation plate. The obtained results suggested that small changes in the ROS levels could have beneficial effects, although further studies are required to fully understand the impact of the extracts and assayed doses on ROS levels to explain the mechanism that is involved in the observed effects.
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spelling pubmed-64066412019-03-19 Antioxidant Characterization and Biological Effects of Grape Pomace Extracts Supplementation in Caenorhabditis elegans Ayuda-Durán, Begoña González-Manzano, Susana Gil-Sánchez, Irene Moreno-Arribas, M. Victoria Bartolomé, Begoña Sanz-Buenhombre, Marisa Guadarrama, Alberto Santos-Buelga, Celestino González-Paramás, Ana M. Foods Article The aim of this work was to evaluate the biological activity of four grape pomace (GP) extracts that are rich in polyphenols using C. elegans as an in vivo model. Different concentrations of the GP extracts were assessed for their effects on the resistance of C. elegans against thermally induced oxidative stress, accumulation of reactive oxygen species (ROS), and lifespan. The cultivation of C. elegans with relatively low concentrations of GP extracts increased their resistance against thermal stress and prolonged their lifespan, while high levels displayed detrimental effects. In the studied extracts, maximum protection was observed for levels of polyphenols around 7 to 9 µg gallic acid equivalents per cultivation plate. The obtained results suggested that small changes in the ROS levels could have beneficial effects, although further studies are required to fully understand the impact of the extracts and assayed doses on ROS levels to explain the mechanism that is involved in the observed effects. MDPI 2019-02-15 /pmc/articles/PMC6406641/ /pubmed/30781355 http://dx.doi.org/10.3390/foods8020075 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ayuda-Durán, Begoña
González-Manzano, Susana
Gil-Sánchez, Irene
Moreno-Arribas, M. Victoria
Bartolomé, Begoña
Sanz-Buenhombre, Marisa
Guadarrama, Alberto
Santos-Buelga, Celestino
González-Paramás, Ana M.
Antioxidant Characterization and Biological Effects of Grape Pomace Extracts Supplementation in Caenorhabditis elegans
title Antioxidant Characterization and Biological Effects of Grape Pomace Extracts Supplementation in Caenorhabditis elegans
title_full Antioxidant Characterization and Biological Effects of Grape Pomace Extracts Supplementation in Caenorhabditis elegans
title_fullStr Antioxidant Characterization and Biological Effects of Grape Pomace Extracts Supplementation in Caenorhabditis elegans
title_full_unstemmed Antioxidant Characterization and Biological Effects of Grape Pomace Extracts Supplementation in Caenorhabditis elegans
title_short Antioxidant Characterization and Biological Effects of Grape Pomace Extracts Supplementation in Caenorhabditis elegans
title_sort antioxidant characterization and biological effects of grape pomace extracts supplementation in caenorhabditis elegans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406641/
https://www.ncbi.nlm.nih.gov/pubmed/30781355
http://dx.doi.org/10.3390/foods8020075
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