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Antioxidant Characterization and Biological Effects of Grape Pomace Extracts Supplementation in Caenorhabditis elegans
The aim of this work was to evaluate the biological activity of four grape pomace (GP) extracts that are rich in polyphenols using C. elegans as an in vivo model. Different concentrations of the GP extracts were assessed for their effects on the resistance of C. elegans against thermally induced oxi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406641/ https://www.ncbi.nlm.nih.gov/pubmed/30781355 http://dx.doi.org/10.3390/foods8020075 |
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author | Ayuda-Durán, Begoña González-Manzano, Susana Gil-Sánchez, Irene Moreno-Arribas, M. Victoria Bartolomé, Begoña Sanz-Buenhombre, Marisa Guadarrama, Alberto Santos-Buelga, Celestino González-Paramás, Ana M. |
author_facet | Ayuda-Durán, Begoña González-Manzano, Susana Gil-Sánchez, Irene Moreno-Arribas, M. Victoria Bartolomé, Begoña Sanz-Buenhombre, Marisa Guadarrama, Alberto Santos-Buelga, Celestino González-Paramás, Ana M. |
author_sort | Ayuda-Durán, Begoña |
collection | PubMed |
description | The aim of this work was to evaluate the biological activity of four grape pomace (GP) extracts that are rich in polyphenols using C. elegans as an in vivo model. Different concentrations of the GP extracts were assessed for their effects on the resistance of C. elegans against thermally induced oxidative stress, accumulation of reactive oxygen species (ROS), and lifespan. The cultivation of C. elegans with relatively low concentrations of GP extracts increased their resistance against thermal stress and prolonged their lifespan, while high levels displayed detrimental effects. In the studied extracts, maximum protection was observed for levels of polyphenols around 7 to 9 µg gallic acid equivalents per cultivation plate. The obtained results suggested that small changes in the ROS levels could have beneficial effects, although further studies are required to fully understand the impact of the extracts and assayed doses on ROS levels to explain the mechanism that is involved in the observed effects. |
format | Online Article Text |
id | pubmed-6406641 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64066412019-03-19 Antioxidant Characterization and Biological Effects of Grape Pomace Extracts Supplementation in Caenorhabditis elegans Ayuda-Durán, Begoña González-Manzano, Susana Gil-Sánchez, Irene Moreno-Arribas, M. Victoria Bartolomé, Begoña Sanz-Buenhombre, Marisa Guadarrama, Alberto Santos-Buelga, Celestino González-Paramás, Ana M. Foods Article The aim of this work was to evaluate the biological activity of four grape pomace (GP) extracts that are rich in polyphenols using C. elegans as an in vivo model. Different concentrations of the GP extracts were assessed for their effects on the resistance of C. elegans against thermally induced oxidative stress, accumulation of reactive oxygen species (ROS), and lifespan. The cultivation of C. elegans with relatively low concentrations of GP extracts increased their resistance against thermal stress and prolonged their lifespan, while high levels displayed detrimental effects. In the studied extracts, maximum protection was observed for levels of polyphenols around 7 to 9 µg gallic acid equivalents per cultivation plate. The obtained results suggested that small changes in the ROS levels could have beneficial effects, although further studies are required to fully understand the impact of the extracts and assayed doses on ROS levels to explain the mechanism that is involved in the observed effects. MDPI 2019-02-15 /pmc/articles/PMC6406641/ /pubmed/30781355 http://dx.doi.org/10.3390/foods8020075 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ayuda-Durán, Begoña González-Manzano, Susana Gil-Sánchez, Irene Moreno-Arribas, M. Victoria Bartolomé, Begoña Sanz-Buenhombre, Marisa Guadarrama, Alberto Santos-Buelga, Celestino González-Paramás, Ana M. Antioxidant Characterization and Biological Effects of Grape Pomace Extracts Supplementation in Caenorhabditis elegans |
title | Antioxidant Characterization and Biological Effects of Grape Pomace Extracts Supplementation in Caenorhabditis elegans |
title_full | Antioxidant Characterization and Biological Effects of Grape Pomace Extracts Supplementation in Caenorhabditis elegans |
title_fullStr | Antioxidant Characterization and Biological Effects of Grape Pomace Extracts Supplementation in Caenorhabditis elegans |
title_full_unstemmed | Antioxidant Characterization and Biological Effects of Grape Pomace Extracts Supplementation in Caenorhabditis elegans |
title_short | Antioxidant Characterization and Biological Effects of Grape Pomace Extracts Supplementation in Caenorhabditis elegans |
title_sort | antioxidant characterization and biological effects of grape pomace extracts supplementation in caenorhabditis elegans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406641/ https://www.ncbi.nlm.nih.gov/pubmed/30781355 http://dx.doi.org/10.3390/foods8020075 |
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