CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations

CRISPR-Cas9 is a promising technology for genome editing. Here we use Cas9 nuclease-induced double-strand break DNA (DSB) at the UROS locus to model and correct congenital erythropoietic porphyria. We demonstrate that homology-directed repair is rare compared with NHEJ pathway leading to on-target i...

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Detalles Bibliográficos
Autores principales: Cullot, Grégoire, Boutin, Julian, Toutain, Jérôme, Prat, Florence, Pennamen, Perrine, Rooryck, Caroline, Teichmann, Martin, Rousseau, Emilie, Lamrissi-Garcia, Isabelle, Guyonnet-Duperat, Véronique, Bibeyran, Alice, Lalanne, Magalie, Prouzet-Mauléon, Valérie, Turcq, Béatrice, Ged, Cécile, Blouin, Jean-Marc, Richard, Emmanuel, Dabernat, Sandrine, Moreau-Gaudry, François, Bedel, Aurélie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408493/
https://www.ncbi.nlm.nih.gov/pubmed/30850590
http://dx.doi.org/10.1038/s41467-019-09006-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408493/
https://www.ncbi.nlm.nih.gov/pubmed/30850590
http://dx.doi.org/10.1038/s41467-019-09006-2

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