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Coupling S-adenosylmethionine–dependent methylation to growth: Design and uses

We present a selection design that couples S-adenosylmethionine–dependent methylation to growth. We demonstrate its use in improving the enzyme activities of not only N-type and O-type methyltransferases by 2-fold but also an acetyltransferase of another enzyme category when linked to a methylation...

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Detalles Bibliográficos
Autores principales: Luo, Hao, Hansen, Anne Sofie L., Yang, Lei, Schneider, Konstantin, Kristensen, Mette, Christensen, Ulla, Christensen, Hanne B., Du, Bin, Özdemir, Emre, Feist, Adam M., Keasling, Jay D., Jensen, Michael K., Herrgård, Markus J., Palsson, Bernhard O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411097/
https://www.ncbi.nlm.nih.gov/pubmed/30856169
http://dx.doi.org/10.1371/journal.pbio.2007050