A bacterial display system for effective selection of protein-biotin ligase BirA variants with novel peptide specificity

Biotinylation creates a sensitive and specific tag for purification and detection of target proteins. The E. coli protein-biotin ligase BirA biotinylates a lysine within a synthetic biotin acceptor peptide (AP) and allow for specific tagging of proteins fused to the AP. The approach is not applicabl...

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Detalles Bibliográficos
Autores principales: Granhøj, Jeff, Dimke, Henrik, Svenningsen, Per
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411976/
https://www.ncbi.nlm.nih.gov/pubmed/30858523
http://dx.doi.org/10.1038/s41598-019-40984-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411976/
https://www.ncbi.nlm.nih.gov/pubmed/30858523
http://dx.doi.org/10.1038/s41598-019-40984-x

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