Cargando…
A bacterial display system for effective selection of protein-biotin ligase BirA variants with novel peptide specificity
Biotinylation creates a sensitive and specific tag for purification and detection of target proteins. The E. coli protein-biotin ligase BirA biotinylates a lysine within a synthetic biotin acceptor peptide (AP) and allow for specific tagging of proteins fused to the AP. The approach is not applicabl...
| Autores principales: | , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2019
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411976/ https://www.ncbi.nlm.nih.gov/pubmed/30858523 http://dx.doi.org/10.1038/s41598-019-40984-x |