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Effect of Enzymatic Treatment of Chrysanthemum Indicum Linné Extracts on Lipid Accumulation and Adipogenesis in High-Fat-Diet-Induced Obese Male Mice

Enzyme treatment of the foods and herbs has been used to improve the absorption rate the efficiency of plant extracts by converting the glycosides of the plant into aglycones. In this study, we examined the obesity-inhibitory effect of Chrysanthemum indicum Linné (CI) treated with enzymes such as vi...

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Autores principales: Lee, Ji-Hyun, Moon, Joo-Myung, Kim, Yoon-Hee, Lee, Bori, Choi, Sang-Yong, Song, Bong-Joon, Kim, Dae-Ki, Lee, Young-Mi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412706/
https://www.ncbi.nlm.nih.gov/pubmed/30691060
http://dx.doi.org/10.3390/nu11020269
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author Lee, Ji-Hyun
Moon, Joo-Myung
Kim, Yoon-Hee
Lee, Bori
Choi, Sang-Yong
Song, Bong-Joon
Kim, Dae-Ki
Lee, Young-Mi
author_facet Lee, Ji-Hyun
Moon, Joo-Myung
Kim, Yoon-Hee
Lee, Bori
Choi, Sang-Yong
Song, Bong-Joon
Kim, Dae-Ki
Lee, Young-Mi
author_sort Lee, Ji-Hyun
collection PubMed
description Enzyme treatment of the foods and herbs has been used to improve the absorption rate the efficiency of plant extracts by converting the glycosides of the plant into aglycones. In this study, we examined the obesity-inhibitory effect of Chrysanthemum indicum Linné (CI) treated with enzymes such as viscozyme and tannase, which are highly efficient in converting glycosides to aglycones and then compared with untreated CI extract. The enzyme-treated CI ethanol extract (CIVT) was administered orally at various doses for 7 weeks in the high fat diet (HFD)-fed male mice. CIVT administration reduced the body weights, the food efficiency and the serum levels of lipid metabolism-related biomarkers, such as triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-c) and leptin in the dose-dependent manner but not those high-density lipoprotein cholesterol (HDL-c) and adiponectin. CIVT also reduced considerably the total lipid amount in the liver and the size of adipocytes in the epididymal white adipose tissue (eWAT). CIVT effectively downregulated the adipogenesis-related transcription factors such as peroxisome proliferation activated receptor (PPAR)-γ and CCAAT/enhancer binding protein-α (C/EBP-α) but up-regulated PPAR-α, in the liver and eWAT. In addition, when compared to the enzyme-untreated CI 50% ethanol extract (CIEE), CIVT enhanced the reduction of body weight and lipid accumulation. Moreover, the viscozyme and tannase treatment of CI increased the flavonoid contents of the aglycone form. Therefore, our results support that the enzymatic treatment induced the production of aglycones for potentially suppressing the adipogenesis and lipid accumulation in HFD-fed mice. It suggests that CIVT might be an effective candidate for attenuating the over-weight and its related diseases.
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spelling pubmed-64127062019-04-09 Effect of Enzymatic Treatment of Chrysanthemum Indicum Linné Extracts on Lipid Accumulation and Adipogenesis in High-Fat-Diet-Induced Obese Male Mice Lee, Ji-Hyun Moon, Joo-Myung Kim, Yoon-Hee Lee, Bori Choi, Sang-Yong Song, Bong-Joon Kim, Dae-Ki Lee, Young-Mi Nutrients Article Enzyme treatment of the foods and herbs has been used to improve the absorption rate the efficiency of plant extracts by converting the glycosides of the plant into aglycones. In this study, we examined the obesity-inhibitory effect of Chrysanthemum indicum Linné (CI) treated with enzymes such as viscozyme and tannase, which are highly efficient in converting glycosides to aglycones and then compared with untreated CI extract. The enzyme-treated CI ethanol extract (CIVT) was administered orally at various doses for 7 weeks in the high fat diet (HFD)-fed male mice. CIVT administration reduced the body weights, the food efficiency and the serum levels of lipid metabolism-related biomarkers, such as triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-c) and leptin in the dose-dependent manner but not those high-density lipoprotein cholesterol (HDL-c) and adiponectin. CIVT also reduced considerably the total lipid amount in the liver and the size of adipocytes in the epididymal white adipose tissue (eWAT). CIVT effectively downregulated the adipogenesis-related transcription factors such as peroxisome proliferation activated receptor (PPAR)-γ and CCAAT/enhancer binding protein-α (C/EBP-α) but up-regulated PPAR-α, in the liver and eWAT. In addition, when compared to the enzyme-untreated CI 50% ethanol extract (CIEE), CIVT enhanced the reduction of body weight and lipid accumulation. Moreover, the viscozyme and tannase treatment of CI increased the flavonoid contents of the aglycone form. Therefore, our results support that the enzymatic treatment induced the production of aglycones for potentially suppressing the adipogenesis and lipid accumulation in HFD-fed mice. It suggests that CIVT might be an effective candidate for attenuating the over-weight and its related diseases. MDPI 2019-01-25 /pmc/articles/PMC6412706/ /pubmed/30691060 http://dx.doi.org/10.3390/nu11020269 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Ji-Hyun
Moon, Joo-Myung
Kim, Yoon-Hee
Lee, Bori
Choi, Sang-Yong
Song, Bong-Joon
Kim, Dae-Ki
Lee, Young-Mi
Effect of Enzymatic Treatment of Chrysanthemum Indicum Linné Extracts on Lipid Accumulation and Adipogenesis in High-Fat-Diet-Induced Obese Male Mice
title Effect of Enzymatic Treatment of Chrysanthemum Indicum Linné Extracts on Lipid Accumulation and Adipogenesis in High-Fat-Diet-Induced Obese Male Mice
title_full Effect of Enzymatic Treatment of Chrysanthemum Indicum Linné Extracts on Lipid Accumulation and Adipogenesis in High-Fat-Diet-Induced Obese Male Mice
title_fullStr Effect of Enzymatic Treatment of Chrysanthemum Indicum Linné Extracts on Lipid Accumulation and Adipogenesis in High-Fat-Diet-Induced Obese Male Mice
title_full_unstemmed Effect of Enzymatic Treatment of Chrysanthemum Indicum Linné Extracts on Lipid Accumulation and Adipogenesis in High-Fat-Diet-Induced Obese Male Mice
title_short Effect of Enzymatic Treatment of Chrysanthemum Indicum Linné Extracts on Lipid Accumulation and Adipogenesis in High-Fat-Diet-Induced Obese Male Mice
title_sort effect of enzymatic treatment of chrysanthemum indicum linné extracts on lipid accumulation and adipogenesis in high-fat-diet-induced obese male mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412706/
https://www.ncbi.nlm.nih.gov/pubmed/30691060
http://dx.doi.org/10.3390/nu11020269
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