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Gut Mucosal Proteins and Bacteriome Are Shaped by the Saturation Index of Dietary Lipids

The dynamics of the tripartite relationship between the host, gut bacteria and diet in the gut is relatively unknown. An imbalance between harmful and protective gut bacteria, termed dysbiosis, has been linked to many diseases and has most often been attributed to high-fat dietary intake. However, w...

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Autores principales: Abulizi, Nijiati, Quin, Candice, Brown, Kirsty, Chan, Yee Kwan, Gill, Sandeep K., Gibson, Deanna L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412740/
https://www.ncbi.nlm.nih.gov/pubmed/30781503
http://dx.doi.org/10.3390/nu11020418
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author Abulizi, Nijiati
Quin, Candice
Brown, Kirsty
Chan, Yee Kwan
Gill, Sandeep K.
Gibson, Deanna L.
author_facet Abulizi, Nijiati
Quin, Candice
Brown, Kirsty
Chan, Yee Kwan
Gill, Sandeep K.
Gibson, Deanna L.
author_sort Abulizi, Nijiati
collection PubMed
description The dynamics of the tripartite relationship between the host, gut bacteria and diet in the gut is relatively unknown. An imbalance between harmful and protective gut bacteria, termed dysbiosis, has been linked to many diseases and has most often been attributed to high-fat dietary intake. However, we recently clarified that the type of fat, not calories, were important in the development of murine colitis. To further understand the host-microbe dynamic in response to dietary lipids, we fed mice isocaloric high-fat diets containing either milk fat, corn oil or olive oil and performed 16S rRNA gene sequencing of the colon microbiome and mass spectrometry-based relative quantification of the colonic metaproteome. The corn oil diet, rich in omega-6 polyunsaturated fatty acids, increased the potential for pathobiont survival and invasion in an inflamed, oxidized and damaged gut while saturated fatty acids promoted compensatory inflammatory responses involved in tissue healing. We conclude that various lipids uniquely alter the host-microbe interaction in the gut. While high-fat consumption has a distinct impact on the gut microbiota, the type of fatty acids alters the relative microbial abundances and predicted functions. These results support that the type of fat are key to understanding the biological effects of high-fat diets on gut health.
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spelling pubmed-64127402019-04-09 Gut Mucosal Proteins and Bacteriome Are Shaped by the Saturation Index of Dietary Lipids Abulizi, Nijiati Quin, Candice Brown, Kirsty Chan, Yee Kwan Gill, Sandeep K. Gibson, Deanna L. Nutrients Article The dynamics of the tripartite relationship between the host, gut bacteria and diet in the gut is relatively unknown. An imbalance between harmful and protective gut bacteria, termed dysbiosis, has been linked to many diseases and has most often been attributed to high-fat dietary intake. However, we recently clarified that the type of fat, not calories, were important in the development of murine colitis. To further understand the host-microbe dynamic in response to dietary lipids, we fed mice isocaloric high-fat diets containing either milk fat, corn oil or olive oil and performed 16S rRNA gene sequencing of the colon microbiome and mass spectrometry-based relative quantification of the colonic metaproteome. The corn oil diet, rich in omega-6 polyunsaturated fatty acids, increased the potential for pathobiont survival and invasion in an inflamed, oxidized and damaged gut while saturated fatty acids promoted compensatory inflammatory responses involved in tissue healing. We conclude that various lipids uniquely alter the host-microbe interaction in the gut. While high-fat consumption has a distinct impact on the gut microbiota, the type of fatty acids alters the relative microbial abundances and predicted functions. These results support that the type of fat are key to understanding the biological effects of high-fat diets on gut health. MDPI 2019-02-16 /pmc/articles/PMC6412740/ /pubmed/30781503 http://dx.doi.org/10.3390/nu11020418 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abulizi, Nijiati
Quin, Candice
Brown, Kirsty
Chan, Yee Kwan
Gill, Sandeep K.
Gibson, Deanna L.
Gut Mucosal Proteins and Bacteriome Are Shaped by the Saturation Index of Dietary Lipids
title Gut Mucosal Proteins and Bacteriome Are Shaped by the Saturation Index of Dietary Lipids
title_full Gut Mucosal Proteins and Bacteriome Are Shaped by the Saturation Index of Dietary Lipids
title_fullStr Gut Mucosal Proteins and Bacteriome Are Shaped by the Saturation Index of Dietary Lipids
title_full_unstemmed Gut Mucosal Proteins and Bacteriome Are Shaped by the Saturation Index of Dietary Lipids
title_short Gut Mucosal Proteins and Bacteriome Are Shaped by the Saturation Index of Dietary Lipids
title_sort gut mucosal proteins and bacteriome are shaped by the saturation index of dietary lipids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412740/
https://www.ncbi.nlm.nih.gov/pubmed/30781503
http://dx.doi.org/10.3390/nu11020418
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