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Transcriptomic Analysis of Vibrio parahaemolyticus Reveals Different Virulence Gene Expression in Response to Benzyl Isothiocyanate

Vibrio parahaemolyticus isolated from seafood is a pathogenic microorganism that leads to several acute diseases that are harmful to our health and is frequently transmitted by food. Therefore, there is an urgent need for the control and suppression of this pathogen. In this paper, transcriptional a...

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Autores principales: Song, Jie, Hou, Hong-Man, Wu, Hong-Yan, Li, Ke-Xin, Wang, Yan, Zhou, Qian-Qian, Zhang, Gong-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412943/
https://www.ncbi.nlm.nih.gov/pubmed/30791538
http://dx.doi.org/10.3390/molecules24040761
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author Song, Jie
Hou, Hong-Man
Wu, Hong-Yan
Li, Ke-Xin
Wang, Yan
Zhou, Qian-Qian
Zhang, Gong-Liang
author_facet Song, Jie
Hou, Hong-Man
Wu, Hong-Yan
Li, Ke-Xin
Wang, Yan
Zhou, Qian-Qian
Zhang, Gong-Liang
author_sort Song, Jie
collection PubMed
description Vibrio parahaemolyticus isolated from seafood is a pathogenic microorganism that leads to several acute diseases that are harmful to our health and is frequently transmitted by food. Therefore, there is an urgent need for the control and suppression of this pathogen. In this paper, transcriptional analysis was used to determine the effect of treatment with benzyl isothiocyanate (BITC) extracted from cruciferous vegetables on V. parahaemolyticus and to elucidate the molecular mechanisms underlying the response to BITC. Treatment with BITC resulted in 332 differentially expressed genes, among which 137 genes were downregulated, while 195 genes were upregulated. Moreover, six differentially expressed genes (DEGs) in RNA sequencing studies were further verified by quantitative real-time polymerase chain reaction (qRT-PCR). Genes found to regulate virulence encoded an l-threonine 3-dehydrogenase, a GGDEF family protein, the outer membrane protein OmpV, a flagellum-specific adenosine triphosphate synthase, TolQ protein and VirK protein. Hence, the results allow us to speculate that BITC may be an effective control strategy for inhibiting microorganisms growing in foods.
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spelling pubmed-64129432019-04-09 Transcriptomic Analysis of Vibrio parahaemolyticus Reveals Different Virulence Gene Expression in Response to Benzyl Isothiocyanate Song, Jie Hou, Hong-Man Wu, Hong-Yan Li, Ke-Xin Wang, Yan Zhou, Qian-Qian Zhang, Gong-Liang Molecules Article Vibrio parahaemolyticus isolated from seafood is a pathogenic microorganism that leads to several acute diseases that are harmful to our health and is frequently transmitted by food. Therefore, there is an urgent need for the control and suppression of this pathogen. In this paper, transcriptional analysis was used to determine the effect of treatment with benzyl isothiocyanate (BITC) extracted from cruciferous vegetables on V. parahaemolyticus and to elucidate the molecular mechanisms underlying the response to BITC. Treatment with BITC resulted in 332 differentially expressed genes, among which 137 genes were downregulated, while 195 genes were upregulated. Moreover, six differentially expressed genes (DEGs) in RNA sequencing studies were further verified by quantitative real-time polymerase chain reaction (qRT-PCR). Genes found to regulate virulence encoded an l-threonine 3-dehydrogenase, a GGDEF family protein, the outer membrane protein OmpV, a flagellum-specific adenosine triphosphate synthase, TolQ protein and VirK protein. Hence, the results allow us to speculate that BITC may be an effective control strategy for inhibiting microorganisms growing in foods. MDPI 2019-02-20 /pmc/articles/PMC6412943/ /pubmed/30791538 http://dx.doi.org/10.3390/molecules24040761 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Song, Jie
Hou, Hong-Man
Wu, Hong-Yan
Li, Ke-Xin
Wang, Yan
Zhou, Qian-Qian
Zhang, Gong-Liang
Transcriptomic Analysis of Vibrio parahaemolyticus Reveals Different Virulence Gene Expression in Response to Benzyl Isothiocyanate
title Transcriptomic Analysis of Vibrio parahaemolyticus Reveals Different Virulence Gene Expression in Response to Benzyl Isothiocyanate
title_full Transcriptomic Analysis of Vibrio parahaemolyticus Reveals Different Virulence Gene Expression in Response to Benzyl Isothiocyanate
title_fullStr Transcriptomic Analysis of Vibrio parahaemolyticus Reveals Different Virulence Gene Expression in Response to Benzyl Isothiocyanate
title_full_unstemmed Transcriptomic Analysis of Vibrio parahaemolyticus Reveals Different Virulence Gene Expression in Response to Benzyl Isothiocyanate
title_short Transcriptomic Analysis of Vibrio parahaemolyticus Reveals Different Virulence Gene Expression in Response to Benzyl Isothiocyanate
title_sort transcriptomic analysis of vibrio parahaemolyticus reveals different virulence gene expression in response to benzyl isothiocyanate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412943/
https://www.ncbi.nlm.nih.gov/pubmed/30791538
http://dx.doi.org/10.3390/molecules24040761
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