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Copy-Paste Mutagenesis: A Method for Large-Scale Alteration of Viral Genomes
The cloning of the large DNA genomes of herpesviruses, poxviruses, and baculoviruses as bacterial artificial chromosomes (BAC) in Escherichia coli has opened a new era in viral genetics. Several methods of lambda Red-mediated genome engineering (recombineering) in E. coli have been described, which...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413233/ https://www.ncbi.nlm.nih.gov/pubmed/30791544 http://dx.doi.org/10.3390/ijms20040913 |