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Copy-Paste Mutagenesis: A Method for Large-Scale Alteration of Viral Genomes

The cloning of the large DNA genomes of herpesviruses, poxviruses, and baculoviruses as bacterial artificial chromosomes (BAC) in Escherichia coli has opened a new era in viral genetics. Several methods of lambda Red-mediated genome engineering (recombineering) in E. coli have been described, which...

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Detalles Bibliográficos
Autores principales: Tang, Jiajia, Brixel, Renke, Brune, Wolfram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413233/
https://www.ncbi.nlm.nih.gov/pubmed/30791544
http://dx.doi.org/10.3390/ijms20040913